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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1996-7-2
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pubmed:abstractText |
sigma S is an alternate sigma factor which functions with RNA polymerase to activate transcription of genes that are involved in a number of stress responses, including stationary-phase survival and osmoprotection. The similarity of the sigma S protein to sigma D (Escherichia coli's major sigma factor) in the regions thought to recognize and bind promoter sequences suggests that sigma S- and sigma D-associated RNA polymerases recognize promoter DNA in a similar manner. However, no promoter recognition sequence for sigma S holoenzyme (E sigma S) has been identified. An apparent conservation of cytosine nucleotides was noted in the -35 region of several sigma S-dependent promoters. Site-directed mutagenesis and reporter gene fusions were used to investigate the importance of the -35 cytosine nucleotides for sigma S-dependent transcription. Substitution of cytosine nucleotides for thymidine at the -35 site of the sigma D-dependent proU promoter effectively abolished transcription by E sigma D but allowed E sigma S to direct transcription from the mutant promoter. Inclusion of the sigma D consensus -10 hexamer strengthened transcription by E sigma S, demonstrating that both E sigma D and E sigma S can recognize the same -10 sequences. Conversely, replacement of -35 site cytosine nucleotides with thymidine in the sigma S-dependent osmY promoter reduced transcription by E sigma S and increased transcription by E sigma D. Our data suggest that DNA sequences in the -35 region function as part of a discriminator mechanism to shift transcription between E sigma D and E sigma S.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1094240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1309519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1317380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1324388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1597408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1648559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1693921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1744047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1849609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-1938945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2213883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2259628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2515417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2566601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2649479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2661827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2661828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2684651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2690013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2693211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2949137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2961367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-2995318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3032964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3049539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3052291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3550697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3596251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3887408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-3905768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-6269464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-6344016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-6754089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7476160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7525405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7545940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7708499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7768815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7768826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7768855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-781293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7814331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7859747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-7934831,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8021175,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8022274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8071215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8206842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8218173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8253679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8253685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8270191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8282684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8416901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8421314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8425216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8631665-8475100
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Coenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Periplasmic Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ProU protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Sigma Factor,
http://linkedlifedata.com/resource/pubmed/chemical/osmY protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/sigma factor KatF protein, Bacteria
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2785-93
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pubmed:dateRevised |
2009-11-18
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