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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-7-3
|
| pubmed:abstractText |
Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U-373 MG, and A-172) expressed endogenous mutant p53, and the other three (U-87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
56
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
694-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8630997-Adenoviruses, Human,
pubmed-meshheading:8630997-Apoptosis,
pubmed-meshheading:8630997-Blotting, Western,
pubmed-meshheading:8630997-Cell Division,
pubmed-meshheading:8630997-Cell Line,
pubmed-meshheading:8630997-Flow Cytometry,
pubmed-meshheading:8630997-Gene Expression,
pubmed-meshheading:8630997-Genes, p53,
pubmed-meshheading:8630997-Genetic Vectors,
pubmed-meshheading:8630997-Glioma,
pubmed-meshheading:8630997-Homozygote,
pubmed-meshheading:8630997-Humans,
pubmed-meshheading:8630997-Kinetics,
pubmed-meshheading:8630997-Recombinant Proteins,
pubmed-meshheading:8630997-Time Factors,
pubmed-meshheading:8630997-Transfection,
pubmed-meshheading:8630997-Tumor Cells, Cultured,
pubmed-meshheading:8630997-Tumor Suppressor Protein p53
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Adenovirus-mediated transfer of the p53 gene produces rapid and generalized death of human glioma cells via apoptosis.
|
| pubmed:affiliation |
Department of Neuro-Oncology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|