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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-6-27
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pubmed:abstractText |
The promoter controlling the expression of the transcript encoding the major herpes simplex virus type 1 capsid protein (VP5, UL19) extends only 60 bases or so from a functional Sp1 site at --48 to include a cis-acting element 3' of the transcript start site. In the present communication, we report the generation of recombinant viruses bearing mutations between --6 and + 8 relative to the cap site in the VP5 promoter controlling expression of a reporter gene. Analysis of the effects of these mutations upon reporter gene expression along with the results of protein binding assays demonstrates that this cap transcription element functionally interacts with a cellular protein of a normal size of 40 kDa. Thus, like the strict late UL38 promoter characterized earlier, the late VP5 promoter has the essential properties of a cellular promoter.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-1313912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-1315705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-1426767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-1658372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-2243103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-2854321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-2958845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-3029970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-7659774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-7956027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-7966567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-7966568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-8057455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-8107257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8627715-8394438
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Caps,
http://linkedlifedata.com/resource/pubmed/chemical/UL19 protein, simplexvirus,
http://linkedlifedata.com/resource/pubmed/chemical/VP5 protein, Herpes simplex virus...,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1898-904
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8627715-Animals,
pubmed-meshheading:8627715-Base Sequence,
pubmed-meshheading:8627715-Capsid,
pubmed-meshheading:8627715-Capsid Proteins,
pubmed-meshheading:8627715-Cell Nucleus,
pubmed-meshheading:8627715-Cells, Cultured,
pubmed-meshheading:8627715-Cercopithecus aethiops,
pubmed-meshheading:8627715-DNA, Viral,
pubmed-meshheading:8627715-DNA-Binding Proteins,
pubmed-meshheading:8627715-HeLa Cells,
pubmed-meshheading:8627715-Herpesvirus 1, Human,
pubmed-meshheading:8627715-Humans,
pubmed-meshheading:8627715-Methylation,
pubmed-meshheading:8627715-Molecular Sequence Data,
pubmed-meshheading:8627715-Multigene Family,
pubmed-meshheading:8627715-Nuclear Proteins,
pubmed-meshheading:8627715-Promoter Regions, Genetic,
pubmed-meshheading:8627715-RNA Caps,
pubmed-meshheading:8627715-Rabbits,
pubmed-meshheading:8627715-Skin,
pubmed-meshheading:8627715-Vero Cells,
pubmed-meshheading:8627715-Viral Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
The herpes simplex virus type 1 VP5 promoter contains a cis-acting element near the cap site which interacts with a cellular protein.
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pubmed:affiliation |
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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