|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
14
|
|
pubmed:dateCreated |
1996-6-21
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth member of this family (HuR) that is expressed in a wide variety of cell types. The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs. In the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all three AU motifs are required for maximal binding.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
271
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
8144-51
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:8626503-Amino Acid Sequence,
pubmed-meshheading:8626503-Antigens, Surface,
pubmed-meshheading:8626503-Base Sequence,
pubmed-meshheading:8626503-Binding Sites,
pubmed-meshheading:8626503-Cloning, Molecular,
pubmed-meshheading:8626503-DNA, Complementary,
pubmed-meshheading:8626503-DNA Primers,
pubmed-meshheading:8626503-Gene Expression Regulation,
pubmed-meshheading:8626503-Genes, fos,
pubmed-meshheading:8626503-Genes, myc,
pubmed-meshheading:8626503-Humans,
pubmed-meshheading:8626503-Interleukin-3,
pubmed-meshheading:8626503-Molecular Sequence Data,
pubmed-meshheading:8626503-RNA, Messenger,
pubmed-meshheading:8626503-RNA-Binding Proteins,
pubmed-meshheading:8626503-Sequence Alignment,
pubmed-meshheading:8626503-Sequence Homology, Amino Acid
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
|
|
pubmed:affiliation |
Program in Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
