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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-6-21
|
| pubmed:databankReference | |
| pubmed:abstractText |
The extracellular matrix is important in the regulation of many cellular events of early development including migration, shape change, proliferation and gene expression. In the sea urchin embryo, disruption of the extracellular matrix results in selective defects in each of these events during gastrulation. Here we describe a new molecule of the extracellular matrix in Lytechinus variegatus, referred to as ECM 18, that has several important features. First, antibody interference of ECM 18 results in a profound but reversible inhibition of primary mesenchyme cell organization and endoderm morphogenesis during gastrulation. Second, during gastrulation, ECM 18 mRNA accumulates to highest levels in the invaginating endoderm and the ECM 18 protein deposited in the basal lamina surrounding the archenteron as well as in other areas of the blastocoel wall. Immunolocalization by fluorescence and electron microscopy demonstrates the selective accumulation of ECM 18 in the extracellular matrix. Third, although the mRNA encoding ECM 18 is present throughout development, the protein accumulates only during gastrulation. ECM 18 protein is not detected in eggs or early embryos and analysis of polysome-associated mRNA suggests that at least part of the translational regulation of ECM 18 is at the level of ECM 18 mRNA-polysome formation. Finally, sequence analysis of ECM 18 shows that the protein contains a repeat sequence with a conserved cysteine motif, suggestive of involvement in protein-protein interactions. Thus, ECM 18 appears to be important in mediating select morphogenetic changes during gastrulation and the pattern of its expression in the embryo is unique among the extracellular matrix molecules known in this embryo.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0950-1991
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
122
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
703-13
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8625821-Amino Acid Sequence,
pubmed-meshheading:8625821-Animals,
pubmed-meshheading:8625821-Antibodies,
pubmed-meshheading:8625821-Blotting, Western,
pubmed-meshheading:8625821-DNA, Complementary,
pubmed-meshheading:8625821-Embryo, Nonmammalian,
pubmed-meshheading:8625821-Extracellular Matrix,
pubmed-meshheading:8625821-Extracellular Matrix Proteins,
pubmed-meshheading:8625821-Fluorescent Antibody Technique,
pubmed-meshheading:8625821-Gastrula,
pubmed-meshheading:8625821-Gene Expression Regulation,
pubmed-meshheading:8625821-Gene Library,
pubmed-meshheading:8625821-In Situ Hybridization,
pubmed-meshheading:8625821-Mesoderm,
pubmed-meshheading:8625821-Molecular Sequence Data,
pubmed-meshheading:8625821-Sea Urchins,
pubmed-meshheading:8625821-Sequence Homology, Amino Acid
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
An extracellular matrix molecule that is selectively expressed during development is important for gastrulation in the sea urchin embryo.
|
| pubmed:affiliation |
Department of Molecular and Cell Biology & Biochemistry, Brown University, Providence, RI 02912, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|