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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1996-6-26
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pubmed:abstractText |
We examined the anti-invasive activity of ursolic acid (UA) on the highly metastatic HT1080 human fibrosarcoma cell line. UA reduced tumor cell invasion through a reconstituted basement membrane in a transwell chamber. A significant down-regulation of matrix metalloproteinase-9 [MMP-9; Mr 92,000 gelatinase/type IV collagenase (gelatinase B)] by UA was detected by Northern blot analysis. However, MMP-2 [Mr 72,000 gelatinase/type IV collagenase (gelatinase A)] and membrane-type MMP were constantly expressed, and the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 also was not changed after 3 and 6 days of treatment with UA. Quantitative gelatin-based zymography confirmed a markedly reduced expression of MMP-9 but not MMP-2 after treatment with UA. To confirm the UA-induced down-regulation of MMP-9 expression, we constructed a secreted alkaline phosphatase (SEAP) reporter vector including MMP-9 promoter. After transfection of MMP-9/SEAP reporter vector into HT1080 cells, reduced SEAP activity was detected after treatment with UA. These results suggest that down-regulation of MMP-9 contributes to the anti-invasive activity of UA in HT1080 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/Gelatinases,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/Triterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/ursolic acid
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2281-4
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8625299-Basement Membrane,
pubmed-meshheading:8625299-Collagenases,
pubmed-meshheading:8625299-Depression, Chemical,
pubmed-meshheading:8625299-Enzyme Induction,
pubmed-meshheading:8625299-Fibrosarcoma,
pubmed-meshheading:8625299-Gelatinases,
pubmed-meshheading:8625299-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8625299-Glycoproteins,
pubmed-meshheading:8625299-Humans,
pubmed-meshheading:8625299-Matrix Metalloproteinase 2,
pubmed-meshheading:8625299-Matrix Metalloproteinase 9,
pubmed-meshheading:8625299-Metalloendopeptidases,
pubmed-meshheading:8625299-Neoplasm Invasiveness,
pubmed-meshheading:8625299-Neoplasm Proteins,
pubmed-meshheading:8625299-Promoter Regions, Genetic,
pubmed-meshheading:8625299-Protein Biosynthesis,
pubmed-meshheading:8625299-Proteins,
pubmed-meshheading:8625299-Tissue Inhibitor of Metalloproteinase-2,
pubmed-meshheading:8625299-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:8625299-Triterpenes,
pubmed-meshheading:8625299-Tumor Cells, Cultured
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pubmed:year |
1996
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pubmed:articleTitle |
Anti-invasive activity of ursolic acid correlates with the reduced expression of matrix metalloproteinase-9 (MMP-9) in HT1080 human fibrosarcoma cells.
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pubmed:affiliation |
Department of Molecular Biology, Pusan National University, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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