pubmed-article:8621523 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0008377 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0079633 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0016390 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0264956 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C1708715 | lld:lifeskim |
pubmed-article:8621523 | lifeskim:mentions | umls-concept:C2346689 | lld:lifeskim |
pubmed-article:8621523 | pubmed:issue | 15 | lld:pubmed |
pubmed-article:8621523 | pubmed:dateCreated | 1996-6-20 | lld:pubmed |
pubmed-article:8621523 | pubmed:abstractText | In order to identify novel genes expressed in macrophage-derived foam cells, we used a multigene assay to examine the expression of genes in control versus cholesterol-loaded macrophages. We compared THP-1 macrophages incubated with or without acetylated LDL (acLDL) +/- acyl-CoA:cholesterol O-acyltransferase (ACAT) inhibitor (compound 58035) for 20 h and assessed changes in mRNA of chemokines, growth factors, interleukins, and adhesion molecules. Among 49 genes examined, an increase in mRNA was observed only for interleukin 8 (IL-8) in THP-1 macrophages. Northern analysis confirmed a 3- to 4-fold increase of IL-8 mRNA and an enzyme-linked immunosorbent assay (ELISA) revealed a corresponding increase in IL-8 in conditioned medium. Oxidized LDL (oxLDL) also induced IL-8 mRNA, but native LDL had no effect. 58035 had a moderate effect on IL-8 induction by acLDL. AcLDL-induced IL-8 expression was concentration- and time-dependent. The time course of IL-8 induction paralleled that of cholesterol loading. MCP-1, a chemokine implicated in recruiting monocytes in atherogenesis, was also induced by acLDL. The induction of MCP-1, however, peaked at 1 h after addition of acLDL and returned to basal level by 20 h while IL-8 induction peaked at 8 h and was still 2-fold higher than basal level at 20 h. IL-8 induction was also observed in fresh human monocyte-derived macrophage cells treated with acLDL. Finally, immunohistochemistry and in situ hybridization studies using specimens of human coronary atheromas showed expression of IL-8 mRNA in a macrophage-rich area. We conclude that IL-8 is induced in macrophage foam cells as a response to cholesterol loading. The chemoattractant and/or mitogenic effects of IL-8 on neutrophils, T cells, smooth muscle, or vascular endothelial cells may contribute to the progression and complications of atherosclerosis. | lld:pubmed |
pubmed-article:8621523 | pubmed:language | eng | lld:pubmed |
pubmed-article:8621523 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8621523 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8621523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8621523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8621523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8621523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8621523 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8621523 | pubmed:month | Apr | lld:pubmed |
pubmed-article:8621523 | pubmed:issn | 0021-9258 | lld:pubmed |
pubmed-article:8621523 | pubmed:author | pubmed-author:WinchesterRR | lld:pubmed |
pubmed-article:8621523 | pubmed:author | pubmed-author:WaniHH | lld:pubmed |
pubmed-article:8621523 | pubmed:author | pubmed-author:TabasII | lld:pubmed |
pubmed-article:8621523 | pubmed:author | pubmed-author:TallAA | lld:pubmed |
pubmed-article:8621523 | pubmed:author | pubmed-author:RabbaniL ELE | lld:pubmed |
pubmed-article:8621523 | pubmed:author | pubmed-author:RavalliSS | lld:pubmed |
pubmed-article:8621523 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8621523 | pubmed:day | 12 | lld:pubmed |
pubmed-article:8621523 | pubmed:volume | 271 | lld:pubmed |
pubmed-article:8621523 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8621523 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8621523 | pubmed:pagination | 8837-42 | lld:pubmed |
pubmed-article:8621523 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:meshHeading | pubmed-meshheading:8621523-... | lld:pubmed |
pubmed-article:8621523 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8621523 | pubmed:articleTitle | Interleukin 8 is induced by cholesterol loading of macrophages and expressed by macrophage foam cells in human atheroma. | lld:pubmed |
pubmed-article:8621523 | pubmed:affiliation | Division of Molecular Medicine, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA. | lld:pubmed |
pubmed-article:8621523 | pubmed:publicationType | Journal Article | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8621523 | lld:pubmed |