Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
|
pubmed:dateCreated |
1996-6-14
|
pubmed:abstractText |
We have previously detected by immunofluorescent assay that the cellular localization of nucleophosmin/B23 (NPM) shifts from the nucleolus to the nucleoplasm (NPM-translocation) after exposure of cells to multiple agents. In order to improve the quantification of the NPM-translocation, we have developed a digital imaging technique. Human Lo leukemia cells, MCF-7 breast carcinoma cells, and fresh human leukemia cells were exposed to anthracyclines or actinomycin D for 4 h. The degree of NPM-translocation was determined and presented as the localization index (LI). Control cells had a LI of about 10, which indicates that the majority of NPM was localized in nucleoli. The LI for drug-treated cells decreased in a dosage- and time-dependent manner. The effect of two classes of anthracycline (daunomycin and aclacinomycin A) and different types of intercalators (daunomycin and actinomycin D) had additive effects on induction of NPM-translocation. The imaging procedure was easily applied to fresh leukemia cells, thus providing useful information regarding drug effects on cancer cells.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aclarubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Daunorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/nucleophosmin
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0304-3835
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
27
|
pubmed:volume |
100
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
191-7
|
pubmed:dateRevised |
2007-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8620441-Aclarubicin,
pubmed-meshheading:8620441-Antibiotics, Antineoplastic,
pubmed-meshheading:8620441-Antineoplastic Combined Chemotherapy Protocols,
pubmed-meshheading:8620441-Breast Neoplasms,
pubmed-meshheading:8620441-Cell Nucleolus,
pubmed-meshheading:8620441-Dactinomycin,
pubmed-meshheading:8620441-Daunorubicin,
pubmed-meshheading:8620441-Dose-Response Relationship, Drug,
pubmed-meshheading:8620441-Fluorescent Antibody Technique,
pubmed-meshheading:8620441-Humans,
pubmed-meshheading:8620441-Image Processing, Computer-Assisted,
pubmed-meshheading:8620441-Leukemia, Monocytic, Acute,
pubmed-meshheading:8620441-Nuclear Proteins,
pubmed-meshheading:8620441-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:8620441-Tumor Cells, Cultured
|
pubmed:year |
1996
|
pubmed:articleTitle |
Quantitation of the nucleophosmin/B23-translocation using imaging analysis.
|
pubmed:affiliation |
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|