Linked Life Data

8618786

Source:http://linkedlifedata.com/resource/pubmed/id/8618786

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-6-7
pubmed:abstractText
CTP:phosphocholine cytidylyltransferase (CT) catalyses a rate regulatory step in the de novo synthesis of surfactant phosphatidylcholine (PC). We have previously shown that CT activity increases during late gestation in alveolar type II cells, and that this increase is most pronounced in microsomes. As it is known that CT is activated by lipids, we investigated the lipid activation of CT in fetal type II cells during late gestation. The degree of activation of cytosolic CT by PC/oleic acid (OA) (1:1 molar ratio) vesicles was gestation-dependent (a 3-fold stimulation on d 18 and a 1.5-fold stimulation on d 21). In contrast, microsomal CT activation by PC/OA vesicles (1.5-fold) remained constant with advancing gestation. Lipids extracted from microsomes of fetal type II cells of different gestational ages (d 18-21) did not differ in their ability to activate either cytosolic CT of d 18 or 21 fetal type II cells, purified CT from adult lung, or delipidated purified CT. In contrast, lipids extracted from cytosol of fetal type II cells of different gestational ages (d 18 and 21) differed in their ability to activate either delipidated cytosolic CT of fetal type II cells, or delipidated purified CT from adult lung. Day 21 cytosolic lipids activated CT more than d 18 cytosolic lipids. Both cytosolic and purified CT, when delipidated by acetone/butanol extraction, showed reduced activities. Several lipids were tested for their ability to activate cytosolic CT. Acidic phospholipids and the mixture of PC/OA (1:1) were the strongest stimulators of cytosolic CT activity. We conclude that cytosolic but not microsomal lipids are involved in the developmental activation of cytosolic CT in fetal type II cells at late gestation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0031-3998
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
864-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Regulation of CTP:phosphocholine cytidylyltransferase by cytosolic lipids in rat type II pneumocytes during development.
pubmed:affiliation
Neonatal Research Division, Hospital for Sick Children, University of Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't