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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-5-3
pubmed:abstractText
The published sequence of the herpes simplex virus (HSV) type 2 strain HG52 neurovirulence gene RL1 indicated that, unlike the HSV-1 homologue, the gene contains a 154 bp intron. This intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. By RT-PCR of poly(A)+ RNA, using primers on either side of the proposed intron, we have isolated a partial cDNA clone corresponding to the predicted spliced mRNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
77 ( Pt 3)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
481-4
pubmed:dateRevised
2006-9-22
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The herpes simplex virus type 2 strain HG52 RL1 gene contains a 154 bp intron as predicted from sequence analysis.
pubmed:affiliation
MRC Virology Unit, Institute of Virology, Glasgow, UK.
pubmed:publicationType
Journal Article