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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1996-5-9
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pubmed:databankReference |
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pubmed:abstractText |
We report the cloning of a transcription-associated histone acetyltransferase type A(HAT A). This Tetrahymena enzyme is strikingly homologous to the yeast protein Gcn5, a putative transcriptional adaptor, and we demonstrate that recombinant Gcn5p possesses HAT activity. Both the ciliate enzyme and Gcn5p contain potential active site residues found in other acetyltransferases and a highly conserved bromodomain. The presence of this domain in nuclear A-type HATs, but not in cytoplasmic B-type HATs, suggests a mechanism whereby HAT A is directed to chromatin to facilitate transcriptional activation. These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
843-51
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8601308-Acetylation,
pubmed-meshheading:8601308-Acetyltransferases,
pubmed-meshheading:8601308-Animals,
pubmed-meshheading:8601308-Base Sequence,
pubmed-meshheading:8601308-Chromatin,
pubmed-meshheading:8601308-Cloning, Molecular,
pubmed-meshheading:8601308-Conserved Sequence,
pubmed-meshheading:8601308-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8601308-Genes, Protozoan,
pubmed-meshheading:8601308-Histone Acetyltransferases,
pubmed-meshheading:8601308-Histones,
pubmed-meshheading:8601308-Molecular Sequence Data,
pubmed-meshheading:8601308-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8601308-Sequence Analysis, DNA,
pubmed-meshheading:8601308-Sequence Homology, Amino Acid,
pubmed-meshheading:8601308-Tetrahymena thermophila,
pubmed-meshheading:8601308-Transcriptional Activation,
pubmed-meshheading:8601308-Yeasts
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pubmed:year |
1996
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pubmed:articleTitle |
Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.
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pubmed:affiliation |
Department of Biology, University of Rochester, New York, 14627, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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