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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-5-2
|
| pubmed:databankReference | |
| pubmed:abstractText |
Eukaryotic rRNAs (with the exception of 5S rRNA) are synthesized from a contiguous pre-rRNA precursor by a complex series of processing reactions. Final maturation of yeast 5.8S rRNA involves processing of a 3'-extended, 7S precursor that contains approximately 140 nucleotides of the internal transcribed spacer 2 (ITS2) region. In yeast strains carrying the temperature-sensitive (ts) rrp4-1 mutation, 5.8S rRNA species were observed with 3' extensions of variable length extending up to the 3' end of the 7S pre-rRNA. These 3'-extended 5.8S rRNA species were observed at low levels in rrp4-1 strains under conditions permissive for growth and increased in abundance upon transfer to the nonpermissive temperature. The RRP4 gene was cloned by complementation of the ts growth phenotype of rrp4-1 strains. RRP4 encodes an essential protein of 39-kD predicted molecular mass. Immunoprecipitated Rrp4p exhibited a 3'-->5' exoribonuclease activity in vitro that required RNA with a 3'-terminal hydroxyl group and released nucleoside 5' monophosphates. We conclude that the 7S pre-rRNA is processed to 5.8S rRNA by a 3'-->5' exonuclease activity involving Rrp4p. Homologs of Rrp4p are found in both humans and fission yeast Schizosaccharomyces pombe (43% and 52% identity, respectively), suggesting that the mechanism of 5.8S rRNA 3' end formation has been conserved throughout eukaryotes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Exoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 5.8S,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
502-13
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8600032-Amino Acid Sequence,
pubmed-meshheading:8600032-Base Sequence,
pubmed-meshheading:8600032-Blotting, Northern,
pubmed-meshheading:8600032-Cell Division,
pubmed-meshheading:8600032-Cloning, Molecular,
pubmed-meshheading:8600032-Conserved Sequence,
pubmed-meshheading:8600032-Evolution, Molecular,
pubmed-meshheading:8600032-Exoribonucleases,
pubmed-meshheading:8600032-Fungal Proteins,
pubmed-meshheading:8600032-Gene Expression Regulation, Fungal,
pubmed-meshheading:8600032-Genes, Fungal,
pubmed-meshheading:8600032-Humans,
pubmed-meshheading:8600032-Molecular Sequence Data,
pubmed-meshheading:8600032-Mutation,
pubmed-meshheading:8600032-RNA, Ribosomal, 5.8S,
pubmed-meshheading:8600032-RNA Precursors,
pubmed-meshheading:8600032-RNA Processing, Post-Transcriptional,
pubmed-meshheading:8600032-Saccharomyces cerevisiae,
pubmed-meshheading:8600032-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8600032-Schizosaccharomyces,
pubmed-meshheading:8600032-Sequence Alignment,
pubmed-meshheading:8600032-Temperature
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism.
|
| pubmed:affiliation |
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|