8599974

pubmed:Citation

3

AP-1/NF-E2 motifs found in erythroid transcription control elements are associated with powerful transcription activation and thought to be regulated by the erythroid transcription factor NF-E2. We have studied AP-1/NF-E2 motifs from three different erythroid control elements (5'HS2 of the human beta-globin locus control region [LCR], the porphobilinogen deaminase [PBGD] promoter, and the mouse Band 3 promoter). We find that these AP-1/NF-E2 elements differ both in their ability to bind NF-E2 and their activity in transient assays. Each of the elements is bound by AP-1, but only the 5'HS2 and PBGD sites are bound by NF-E2. We examined the activity of these sites in minimal promoter constructs in transient assays. In erythroid cells, activity of duplicated NF-E2 motifs is positively correlated with binding by NF-E2; however, the Band 3 element not bound by NF-E2 is also active in some contexts. In HeLa cells, all sites were active and duplicated sites were most active. In F9 mouse teratocarcinoma cells, which express neither NF-E2 nor AP-1, the elements' activity parallels that in erythroid cells. While these finding are consistent with other evidence that NF-E2 is an important regulator of erythroid transcription, they suggest that some sites that resemble NF-E2 elements are actually regulated by other factors; we speculate that other tissue-specific and/or generally expressed factors may act on these sites.

eng

IM

MEDLINE

0301-472X

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NLM

445-52

pubmed-meshheading:8599974-Animals, pubmed-meshheading:8599974-Base Sequence, pubmed-meshheading:8599974-Binding, Competitive, pubmed-meshheading:8599974-Binding Sites, pubmed-meshheading:8599974-DNA, pubmed-meshheading:8599974-DNA-Binding Proteins, pubmed-meshheading:8599974-Erythroid-Specific DNA-Binding Factors, pubmed-meshheading:8599974-Globins, pubmed-meshheading:8599974-HeLa Cells, pubmed-meshheading:8599974-Humans, pubmed-meshheading:8599974-Leukemia, Erythroblastic, Acute, pubmed-meshheading:8599974-Mice, pubmed-meshheading:8599974-Molecular Sequence Data, pubmed-meshheading:8599974-NF-E2 Transcription Factor, pubmed-meshheading:8599974-NF-E2 Transcription Factor, p45 Subunit, pubmed-meshheading:8599974-Promoter Regions, Genetic, pubmed-meshheading:8599974-Rabbits, pubmed-meshheading:8599974-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8599974-Transcription Factor AP-1, pubmed-meshheading:8599974-Transcription Factors, pubmed-meshheading:8599974-Tumor Cells, Cultured

Erythroid AP-1/NF-E2 elements vary in their response to NF-E2.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't