|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1996-4-24
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We have analyzed 3.4 kb of DNA from the 5'-flanking region of the rat beta 2-adrenergic receptor gene and assessed its promoter activity in A549 cells, a human lung adenocarcinoma cell line. A single transcription start site was identified approx. 223 bp upstream of the ATG start codon. A549 cells were transfected with luciferase reporter plasmids containing segments of the rat beta 2-adrenergic receptor 5'-flanking region. Our results suggest that both positive and negative cis-acting regulatory sequences are present in the 5'-flanking region of the rat beta 2-adrenergic receptor gene.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0006-3002
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
1305
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
135-8
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:8597598-Adenocarcinoma,
pubmed-meshheading:8597598-Amino Acid Sequence,
pubmed-meshheading:8597598-Animals,
pubmed-meshheading:8597598-Base Sequence,
pubmed-meshheading:8597598-Binding Sites,
pubmed-meshheading:8597598-Cell Line,
pubmed-meshheading:8597598-Codon,
pubmed-meshheading:8597598-DNA,
pubmed-meshheading:8597598-DNA Primers,
pubmed-meshheading:8597598-DNA-Binding Proteins,
pubmed-meshheading:8597598-Humans,
pubmed-meshheading:8597598-Luciferases,
pubmed-meshheading:8597598-Lung Neoplasms,
pubmed-meshheading:8597598-Molecular Sequence Data,
pubmed-meshheading:8597598-Molecular Weight,
pubmed-meshheading:8597598-Promoter Regions, Genetic,
pubmed-meshheading:8597598-Rats,
pubmed-meshheading:8597598-Receptors, Adrenergic, beta-2,
pubmed-meshheading:8597598-Recombinant Proteins,
pubmed-meshheading:8597598-Restriction Mapping,
pubmed-meshheading:8597598-TATA Box,
pubmed-meshheading:8597598-Transcription, Genetic,
pubmed-meshheading:8597598-Transfection,
pubmed-meshheading:8597598-Tumor Cells, Cultured
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Structural and functional analysis of the 5'-flanking region of the rat beta 2-adrenergic receptor gene.
|
|
pubmed:affiliation |
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, 72205, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|
