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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-4-15
|
| pubmed:abstractText |
We generated mice transgenic for a VH gene that partially encodes an anti-IgG2a rheumatoid factor. Such transgenic VH genes recombine at a low frequency with the endogenous Igh locus in mice, giving rise to a small number of B cells that express heavy chains partially encoded by the transgene. The transgenes were crossed onto an lpr/lpr background, and hybridomas were generated from the resulting mice at 3 to 6 mo of age. Analysis of the anti-IgG2a- producing hybridomas obtained revealed that none expressed the transgenic VH. Surprisingly, however, most of the mice yielded multiple anti-IgG2a hybridomas that expressed VH genes comprised of a single VH gene segment, D regions with highly homologous 5' ends encoding CDR3 regions of identical length, and the JH4 segment. Expressed light chain diversity among these hybridomas was also highly restricted; most expressed a single V kappa gene segment. All of the hybridomas expressed members of the V kappa 19/28 family. Many of the VH genes contained a low frequency of somatic mutation. The recurrence of this family of V regions is not due to an indirect transgene effect or to effects of the genetic background used to construct the mice, as hybridomas expressing the predominant V gene segment combination were also isolated from a transgene-negative lpr/lpr littermate and from MRL lpr/lpr mice. These data contrast with the previous findings of others that while the spontaneous rheumatoid factor response of lpr/lpr mice was oligoclonal, recurrent clonotypes were not apparent, and the VH and V kappas encoding these rheumatoid factors contained a high frequency of somatic mutation whose distribution and type were indicative of Ag-driven selection.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Rheumatoid Factor,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgG
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
156
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1856-64
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8596037-Animals,
pubmed-meshheading:8596037-Antibodies, Anti-Idiotypic,
pubmed-meshheading:8596037-Base Sequence,
pubmed-meshheading:8596037-Clone Cells,
pubmed-meshheading:8596037-Female,
pubmed-meshheading:8596037-Genes, Immunoglobulin,
pubmed-meshheading:8596037-Immunoglobulin G,
pubmed-meshheading:8596037-Immunoglobulin Heavy Chains,
pubmed-meshheading:8596037-Immunoglobulin Variable Region,
pubmed-meshheading:8596037-Male,
pubmed-meshheading:8596037-Mice,
pubmed-meshheading:8596037-Mice, Inbred C3H,
pubmed-meshheading:8596037-Mice, Inbred C57BL,
pubmed-meshheading:8596037-Mice, Mutant Strains,
pubmed-meshheading:8596037-Mice, Transgenic,
pubmed-meshheading:8596037-Molecular Sequence Data,
pubmed-meshheading:8596037-Rheumatoid Factor,
pubmed-meshheading:8596037-Spleen
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A recurrent clonotype in the spontaneous anti-IgG2a rheumatoid factor response of lpr/lpr mice.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Thomas Jefferson Medical College, Philadelphia, PA 19017, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|