Linked Life Data

8590056

Source:http://linkedlifedata.com/resource/pubmed/id/8590056

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-3-25
pubmed:abstractText
Free amino acid composition of cerebrospinal fluid (CSF) in the rat was measured to examine if electrical activity of neurons in the ventromedial nucleus of the hypothalamus (VMH) is modulated by amino acids at CSF levels. In CSF collected through a cannula in the cerebromedullary cistern of freely moving rats, the total concentration of amino acids was 979 microM, while that in plasma was 3.50 mM. Glutamine was the major (60%) component and each of other amino acids was less than 8.0% (lysine) of total. Among neutral amino acids, concentrations of serine, threonine and alanine were much higher than those of others. Although concentrations of basic amino acids such as lysine and arginine were also relatively high, those of acidic amino acids such as glutamate and aspartate were very low. Spontaneous firing rates of 73% of the neurons in the VMH in brain slices in vitro were facilitated by changing the perfusion medium from artificial CSF (ACSF) without amino acids to one containing each amino acid close to CSF levels (AA-ACSF). Glutamine (600 microM) alone similarly facilitated the activity in 64% of VMH neurons, though the facilitatory effect of amino acids was blocked by simultaneous application of neither AP-5 nor CNQX, antagonists for NMDA or non-NMDA receptors, respectively, to AA-ACSF at 100 microM.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
691
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Excitability of neurons in the ventromedial nucleus in rat hypothalamic slices: modulation by amino acids at cerebrospinal fluid levels.
pubmed:affiliation
Department of Veterinary Physiology, Veterinary Medical Science, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't