Linked Life Data

8589932

Source:http://linkedlifedata.com/resource/pubmed/id/8589932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1996-3-27
pubmed:databankReference
pubmed:abstractText
Vacuolar processing enzymes (VPEs) are responsible for the maturation of seed proteins. Southern blot analysis showed that a family of genes for VPEs in Arabidopsis thaliana was composed of three genes, for alpha-VPE, beta-VPE and gamma-VPE, respectively. The gene for gamma-VPE was isolated from a genomic library. It was composed of nine exons and eight introns. The positions of the introns were fully conserved among the three genes, with the exception that the alpha-VPE gene was missing the fifth intron found in the beta-VPE and gamma-VPE genes. The predicted gamma-VPE protein was 80% and 57% identical in terms of amino acid sequence to the alpha-VPE protein and beta-VPE protein, respectively. Northern blot analysis demonstrated that the gamma-VPE gene was expressed predominantly in the stems, with a lower level of expression in rosette and cauline leaves. However, the expression was not detected in roots, flowers plus buds, or green siliques, in contrast to the high-level expression of the beta-VPE gene in the flowers plus buds. Thus, gamma-VPE seems to be an isoform that is specific to vegetative organs. Members of the VPE family can be separated into two subfamilies, one that is specific to seeds and another that is specific to vegetative organs, such as leaves and stems. The members of the seed subfamily might function in the protein-storage vacuoles of seeds, while those of the vegetable subfamily might function in the lytic vacuoles of non-storage organs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0032-0781
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1555-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
The sequence and expression of the gamma-VPE gene, one member of a family of three genes for vacuolar processing enzymes in Arabidopsis thaliana.
pubmed:affiliation
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't