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rdf:type |
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lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0007634,
umls-concept:C0013081,
umls-concept:C0031586,
umls-concept:C0087111,
umls-concept:C0205225,
umls-concept:C0205374,
umls-concept:C0443199,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
6
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pubmed:dateCreated |
1996-3-25
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pubmed:abstractText |
We studied cAMP responses induced by parathyroid hormone (PTH), prostaglandin E2 (PGE2) and forskolin in foetal rat calvariae-derived osteoblastic cells after 24 h treatment with a protein kinase C (PKC) activating phorbol ester. After this treatment, meant to down-regulate PKC activity, all tested cAMP responses were attenuated and were indeed accompanied by a decline in PKC activity. PTH receptor affinity was not altered and PTH receptor number was only slightly lowered after 24 h phorbol ester treatment. These results indicate that modulation of the cAMP responses by 24 h PMA treatment was mainly caused by a general impairment of adenylyl cyclase activity. Removal of the phorbol ester and subsequent culture for 2 days rendered the cells hyper-responsive to PTH: the PTH-induced cAMP response was 2 to 3 times higher than in control cells. Again no change in binding affinity of the PTH receptor was observed and receptor number was just 10% lower than in control cells. The PGE2- and forskolin-induced cAMP responses were not higher than normal. So, transient phorbol ester treatment leads to a differential, agonist-dependent restoration of the cAMP signalling system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Parathyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Parathyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/phorbol-12,13-didecanoate
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0898-6568
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
617-26
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8588978-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:8588978-Adenylate Cyclase,
pubmed-meshheading:8588978-Animals,
pubmed-meshheading:8588978-Cells, Cultured,
pubmed-meshheading:8588978-Cyclic AMP,
pubmed-meshheading:8588978-Dinoprostone,
pubmed-meshheading:8588978-Down-Regulation,
pubmed-meshheading:8588978-Enzyme Activation,
pubmed-meshheading:8588978-Forskolin,
pubmed-meshheading:8588978-Kinetics,
pubmed-meshheading:8588978-Osteoblasts,
pubmed-meshheading:8588978-Parathyroid Hormone,
pubmed-meshheading:8588978-Phorbol Esters,
pubmed-meshheading:8588978-Phosphodiesterase Inhibitors,
pubmed-meshheading:8588978-Protein Kinase C,
pubmed-meshheading:8588978-Rats,
pubmed-meshheading:8588978-Receptors, Parathyroid Hormone,
pubmed-meshheading:8588978-Signal Transduction,
pubmed-meshheading:8588978-Skull,
pubmed-meshheading:8588978-Tetradecanoylphorbol Acetate
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pubmed:year |
1995
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pubmed:articleTitle |
Down-regulation and differential restoration of cAMP responses upon transient phorbol ester treatment of primary osteoblastic cells.
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pubmed:affiliation |
Laboratory of Cell Biology and Histology, University of Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article
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