Linked Life Data

8580685

Source:http://linkedlifedata.com/resource/pubmed/id/8580685

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-3-20
pubmed:abstractText
Paracoccidioides brasiliensis (Pb) is the dimorphic fungus responsible for paracoccidioidomycosis (PCM), one of the most important systemic mycosis in Latin America where the disease is geographically restricted. The natural habitat of the causative agent remains undetermined. We are planning to use PCR-based technology in order to amplify specific DNA fragments. The high sensitivity of this technique may allow us to detect the natural habitat of Pb. In this study, we prepared a cDNA library from which we cloned a protein of approximately 27 kDa MW. When this recombinant antigenic protein was tested by the immunoblot technique, it was able to recognize antibodies in the sera of 91% of the PCM patients studied. No cross reactions were observed with sera from patients with other systemic mycoses. Presently we are sequencing and characterizing this clone, in order to design specific primers for amplification of Pb DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0188-4409
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-6
pubmed:dateRevised
2005-11-16
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
In search of the natural habitat of Paracoccidioides brasiliensis.
pubmed:affiliation
Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.
pubmed:publicationType
Journal Article, Review