Linked Life Data

8580083

Source:http://linkedlifedata.com/resource/pubmed/id/8580083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1996-3-18
pubmed:abstractText
Protein tyrosine kinase p59fyn (Fyn) associates with the TCR-CD3 complex, which suggests that Fyn plays a significant role in the signal transduction involving TCR complex. In addition to cellular genes, viral promoters such as the HIV long terminal repeat (LTR) are also activated upon T cell activation. To elucidate the functional significance of Fyn in the expression of viral promoters, we transfected a Fyn-expression vector together with a reporter plasmid containing the chloramphenicol acetyltransferase gene driven by HIV LTR into a human T cell line, Jurkat. In this assay, Fyn stimulated the promoter in HIV LTR when the transfected cells were treated with both concanavalin A and PMA as an antigen-mimic stimulation. This activation required the intact SH2 domain of Fyn. Mutational analysis of HIV LTR showed that the NF kappa B binding sites were responsible for this effect. Electrophoretic mobility shift assays and UV cross-linking experiments showed that activation of T cells by anti-CD3 antibody induced four kappa B-binding proteins (50, 60, 65 and 100 kDa) in Fyn-overexpressing cells more efficiently than in the parental cells. Our results suggested that Fyn was able to regulate expression of a subset of genes via kappa B-binding proteins upon T cell activation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1851-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8580083-Base Sequence, pubmed-meshheading:8580083-Binding Sites, pubmed-meshheading:8580083-DNA-Binding Proteins, pubmed-meshheading:8580083-Gene Expression Regulation, pubmed-meshheading:8580083-HIV, pubmed-meshheading:8580083-Humans, pubmed-meshheading:8580083-Leukemia, T-Cell, pubmed-meshheading:8580083-Molecular Sequence Data, pubmed-meshheading:8580083-NF-kappa B, pubmed-meshheading:8580083-Promoter Regions, Genetic, pubmed-meshheading:8580083-Protein-Tyrosine Kinases, pubmed-meshheading:8580083-Proto-Oncogene Proteins, pubmed-meshheading:8580083-Proto-Oncogene Proteins c-fyn, pubmed-meshheading:8580083-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:8580083-Transcription, Genetic, pubmed-meshheading:8580083-Transcriptional Activation, pubmed-meshheading:8580083-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
The protein tyrosine kinase Fyn activates transcription from the HIV promoter via activation of NF kappa B-like DNA-binding proteins.
pubmed:affiliation
Department of Oncology, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't