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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-3-11
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pubmed:abstractText |
C-peptide radioimmunoassay (C-peptide RIA) is widely used in determination of pancreatic B-cell secretion activity. 125I labeled Tyr-C-peptide is indispensable in C-peptide RIA kit. Herein we discuss a way of obtaining recombinant Tyr-C-peptide. Arg32Tyr human pro-insulin mutant (R32Y-proinsulin) gene was constructed by site-directed mutagenesis and overexpressed in Escherichia coli. Purified R32Y-proinsulin was converted to insulin and Tyr-C-peptide by trypsin and carboxypeptidase B codigestion. Tyr-C-peptide was isolated through reverse-phase HPLC (RP-HPLC) and identified by C-peptide RIA and amino acid analysis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0273-2289
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-74
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:8579343-Base Sequence,
pubmed-meshheading:8579343-C-Peptide,
pubmed-meshheading:8579343-Carboxypeptidase B,
pubmed-meshheading:8579343-Carboxypeptidases,
pubmed-meshheading:8579343-Chromatography, High Pressure Liquid,
pubmed-meshheading:8579343-DNA Primers,
pubmed-meshheading:8579343-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8579343-Escherichia coli,
pubmed-meshheading:8579343-Gene Expression Regulation,
pubmed-meshheading:8579343-Humans,
pubmed-meshheading:8579343-Molecular Sequence Data,
pubmed-meshheading:8579343-Mutagenesis, Site-Directed,
pubmed-meshheading:8579343-Plasmids,
pubmed-meshheading:8579343-Proinsulin,
pubmed-meshheading:8579343-Radioimmunoassay,
pubmed-meshheading:8579343-Recombinant Proteins,
pubmed-meshheading:8579343-Restriction Mapping,
pubmed-meshheading:8579343-Trypsin,
pubmed-meshheading:8579343-Tyrosine
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pubmed:year |
1995
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pubmed:articleTitle |
Preparation of Tyr-C-peptide from genetically altered human insulin precursor.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing, China.
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pubmed:publicationType |
Journal Article
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