Linked Life Data

8572584

Source:http://linkedlifedata.com/resource/pubmed/id/8572584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5B
pubmed:dateCreated
1996-3-1
pubmed:abstractText
Phospholipase A2 (PLA2) generated lipid biomediators can facilitate neoplastic progression. Specific PLA2 alterations associated with ras oncogene expression were determined by comparison of PLA2 activities in nontumorigenic rat embryo fibroblasts (CREF cells) and their tumorigenic ras oncogene-transfected counterparts (CREF-T24 cells). The high molecular mass cytosolic PLA2 activity is 2-3 fold higher in CREF-T24 cells as compared to CREF cells. Western blotting analyses indicate increases in the level of this enzyme and the proportion which migrates with phosphorylated enzyme in CREF-T24 cells. A PLA2 activity, with the characteristics of a group II PLA2, is readily detectible in particulate fractions from CREF-T24 cells following ammonium sulfate extraction/cation ion exchange chromatography. In contrast, this activity is minimal in similarly prepared CREF cell samples. While the CREF-T24 cells have increases in two PLA2 activities associated with the release of arachidonic acid, the CREF-T24 and CREF cells are similar with respect to Ca2+ independent, particulate fraction-associated PLA2 activities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1957-62
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:articleTitle
Phospholipases A2 in ras-transfected fibroblasts.
pubmed:affiliation
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't