Linked Life Data

8572241

Source:http://linkedlifedata.com/resource/pubmed/id/8572241

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 1
pubmed:dateCreated
1996-3-7
pubmed:abstractText
The goal of this study was to determine the temporal and spatial sequence of events that accompany lung injury and repair after parenteral administration of the Clara cell-specific cytotoxicant, naphthalene. Changes in airway epithelial cells were evaluated by measuring alterations in the expression of markers for differentiated Clara cells (CYPIIF and Clara cell 10-kDa secretory protein, CC10), distal airway/alveolar type II cells (surfactant protein B; SP-B) and for cycling/proliferating cells (cyclin dependent kinase 1;CDK1). Naphthalene-induced Clara cell cytotoxicity resulted in the exfoliation of epithelial cells containing CC10 protein. This was accompanied by a dramatic reduction in the abundance of mRNA for CC10 and CYPIIF. Large numbers of CDK1 mRNA-positive cells were identified in and around bronchioles and terminal bronchioles 48 h after treatment. This cellular proliferation resulted in the population of airways by immature epithelial cells lacking normal levels of CC10 mRNA but overexpressing SP-B mRNA. Seventy-two hours after naphthalene treatment a reduction in CDK1 mRNA-positive cells was noted within bronchioles and terminal bronchioles at all locations, with the exception of airway bifurcations. At airway bifurcations CDK1 mRNA appeared to be more abundant at the 72-h time point than at 48 h. Comparison of these sections with serial sections probed for CC10 mRNA demonstrated a correlation between the expression of CDK1 and CC10 mRNA at bifurcations. Temporal increases in the abundance of CC10 mRNA observed at later time points were largely accounted for by the processive maturation of newly repopulated cells neighboring bifurcations in bronchioles. These studies identify spatially distinct populations of cells that act in concert to repopulate naphthalene-injured airways and support the notion that branch point cells play an important role in the maturation of newly regenerated airway epithelial cells after acute injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L791-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8572241-Animals, pubmed-meshheading:8572241-Cell Division, pubmed-meshheading:8572241-Cytochrome P-450 Enzyme System, pubmed-meshheading:8572241-Dose-Response Relationship, Drug, pubmed-meshheading:8572241-Female, pubmed-meshheading:8572241-Gene Expression, pubmed-meshheading:8572241-Immunohistochemistry, pubmed-meshheading:8572241-In Situ Hybridization, pubmed-meshheading:8572241-Lung, pubmed-meshheading:8572241-Mice, pubmed-meshheading:8572241-Mice, Inbred Strains, pubmed-meshheading:8572241-Naphthalenes, pubmed-meshheading:8572241-Oxygenases, pubmed-meshheading:8572241-Proteins, pubmed-meshheading:8572241-Proteolipids, pubmed-meshheading:8572241-Pulmonary Surfactants, pubmed-meshheading:8572241-RNA, Messenger, pubmed-meshheading:8572241-Time Factors, pubmed-meshheading:8572241-Tissue Distribution, pubmed-meshheading:8572241-Uteroglobin, pubmed-meshheading:8572241-Wound Healing
pubmed:year
1995
pubmed:articleTitle
Plasticity of airway cell proliferation and gene expression after acute naphthalene injury.
pubmed:affiliation
Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't