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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-2-23
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pubmed:abstractText |
NF-kappa B is a potent inducible transcription factor that regulates many genes in activated T cells. In this report we examined the ability of different subunits of NF-kappa B to enhance HIV-1 transcription in vitro with chromatin templates. We find that the p65 subunit of NF-kappa B is a strong transcriptional activator of nucleosome-assembled HIV-1 DNA, whereas p50 does not activate transcription, and that p65 activates transcription synergistically with Sp1 and distal HIV-1 enhancer-binding factors (LEF-1, Ets-1, and TFE-3). These effects were observed with chromatin, but not with nonchromatin templates. Furthermore, binding of either p50 or p65 with Sp1 induces rearrangement of the chromatin to a structure that resembles the one reported previously for integrated HIV-1 proviral DNA in vivo. These results suggest that p50 and Sp1 contribute to the establishment of the nucleosomal arrangement of the uninduced provirus in resting T cells, and that p65 activates transcription by recruitment of the RNA polymerase II transcriptional machinery to the chromatin-repressed basal promoter.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LEF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphoid Enhancer-Binding Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B p50 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleosomes,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor RelA,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-49
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8557193-Base Sequence,
pubmed-meshheading:8557193-Binding Sites,
pubmed-meshheading:8557193-Chromatin,
pubmed-meshheading:8557193-DNA-Binding Proteins,
pubmed-meshheading:8557193-Gene Expression Regulation, Viral,
pubmed-meshheading:8557193-HIV Enhancer,
pubmed-meshheading:8557193-HIV Long Terminal Repeat,
pubmed-meshheading:8557193-HeLa Cells,
pubmed-meshheading:8557193-Humans,
pubmed-meshheading:8557193-Lymphoid Enhancer-Binding Factor 1,
pubmed-meshheading:8557193-Molecular Sequence Data,
pubmed-meshheading:8557193-NF-kappa B,
pubmed-meshheading:8557193-NF-kappa B p50 Subunit,
pubmed-meshheading:8557193-Nucleosomes,
pubmed-meshheading:8557193-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8557193-Sp1 Transcription Factor,
pubmed-meshheading:8557193-Trans-Activators,
pubmed-meshheading:8557193-Transcription, Genetic,
pubmed-meshheading:8557193-Transcription Factor RelA,
pubmed-meshheading:8557193-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
NF-kappa B-mediated chromatin reconfiguration and transcriptional activation of the HIV-1 enhancer in vitro.
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pubmed:affiliation |
Department of Biology, University of California, San Diego (UCSD), La Jolla 92093-0347, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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