Linked Life Data

8552644

Source:http://linkedlifedata.com/resource/pubmed/id/8552644

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-2-22
pubmed:abstractText
Orally administered antigens induce a state of immunologic hyporesponsiveness termed oral tolerance. Different mechanisms are involved in mediating oral tolerance depending on the dose fed. Low doses of antigen generate cytokine-secreting regulatory cells, whereas high doses induce anergy or deletion. We used mice transgenic for a T-cell receptor (TCR) derived from an encephalitogenic T-cell clone specific for the acetylated N-terminal peptide of myelin basic protein (MBP) Ac-1-11 plus I-Au to test whether a regulatory T cell could be generated from the same precursor cell as that of an encephalitogenic Th1 cell and whether the induction was dose dependent. The MBP TCR transgenic mice primarily have T cells of a precursor phenotype that produce interleukin 2 (IL-2) with little interferon gamma (IFN-gamma), IL-4, or transforming growth factor beta (TGF-beta). We fed transgenic animals a low-dose (1 mg x 5) or high-dose (25 mg x 1) regimen of mouse MBP and without further immunization spleen cells were tested for cytokine production. Low-dose feeding induced prominent secretion of IL-4, IL-10, and TGF-beta, whereas minimal secretion of these cytokines was observed with high-dose feeding. Little or no change was seen in proliferation or IL-2/IFN-gamma secretion in fed animals irrespective of the dose. To demonstrate in vivo functional activity of the cytokine-secreting cells generated by oral antigen, spleen cells from low-dose-fed animals were adoptively transferred into naive (PLJ x SJL)F1 mice that were then immunized for the development of experimental autoimmune encephalomyelitis (EAE). Marked suppression of EAE was observed when T cells were transferred from MBP-fed transgenic animals but not from animals that were not fed. In contrast to oral tolerization, s.c. immunization of transgenic animals with MBP in complete Freund's adjuvant induced IFN-gamma-secreting Th1 cells in vitro and experimental encephalomyelitis in vivo. Despite the large number of cells reactive to MBP in the transgenic animals, EAE was also suppressed by low-dose feeding of MBP prior to immunization. These results demonstrate that MBP-specific T cells can differentiate in vivo into encephalitogenic or regulatory T cells depending upon the context by which they are exposed to antigen.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-1370356, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-1548698, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-2025413, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-2190093, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-2474183, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-2611224, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-3709525, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-6251047, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-6667333, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-6972088, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7520367, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7520605, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7530361, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7603570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7664081, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7678116, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7693817, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7744081, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7877175, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-7925432, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8011298, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8022835, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8255292, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8262237, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8352587, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8369276, http://linkedlifedata.com/resource/pubmed/commentcorrection/8552644-8381670
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
388-91
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Oral tolerance in myelin basic protein T-cell receptor transgenic mice: suppression of autoimmune encephalomyelitis and dose-dependent induction of regulatory cells.
pubmed:affiliation
Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.