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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0033713,
umls-concept:C0185117,
umls-concept:C0332281,
umls-concept:C0332291,
umls-concept:C0812215,
umls-concept:C1155065,
umls-concept:C1155437,
umls-concept:C1706586,
umls-concept:C2746015,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1996-2-14
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pubmed:abstractText |
We have studied pim-1 proto-oncogene expression in human T cell responses to Ag receptor-generated signals. The pim-1 gene encodes a serine/threonine protein kinase that is expressed primarily in cells of hematopoietic lineage and is implicated in the intracellular signaling processes accompanying lymphocyte activation. We show here that pim-1 mRNA expression is rapidly induced after receptor cross-linking with anti-CD3 Abs. We examined the linkage of pim-1 expression to known signaling pathways generated through the T cell Ag receptor. pim-1 mRNA was not substantially induced after elevation of intracellular free Ca2+. In contrast, PMA, which directly activates PKC, induced rapid pim-1 expression. Further, anti-CD3- or PMA-induced pim-1 expression was markedly reduced by various PKC inhibitors and by deficiency of the PKC epsilon isoform in a mutant T cell line. Thus, T cell Ag receptor-linked pim-1 expression appears to be coupled to the PKC component of transmembrane signaling. Because the activation of protein kinase C has been shown to activate Raf-1 kinase activity, the involvement of Raf-1 in pim-1 expression was also investigated using a human T cell line stably transfected with an inducible Raf expression vector. Although the overexpression of activated Raf was shown to cause a substantial increase in IL-2 expression, no discernible effects on pim-1 were apparent. In addition, we examined transcriptional and post-transcriptional mechanisms involved in PKC-mediated pim-1 expression and observed that both transcriptional and post-transcriptional mechanisms are coordinately involved in the up-regulation of the pim-1 proto-oncogene.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Muromonab-CD3,
http://linkedlifedata.com/resource/pubmed/chemical/PIM1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-pim-1,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
156
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
549-57
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:8543805-Calcium,
pubmed-meshheading:8543805-Cell Nucleus,
pubmed-meshheading:8543805-Cytoplasm,
pubmed-meshheading:8543805-Enzyme Activation,
pubmed-meshheading:8543805-Enzyme Induction,
pubmed-meshheading:8543805-Humans,
pubmed-meshheading:8543805-Ionomycin,
pubmed-meshheading:8543805-Isoenzymes,
pubmed-meshheading:8543805-Leukemia-Lymphoma, Adult T-Cell,
pubmed-meshheading:8543805-Lymphocyte Activation,
pubmed-meshheading:8543805-Muromonab-CD3,
pubmed-meshheading:8543805-Protein Kinase C,
pubmed-meshheading:8543805-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8543805-Proto-Oncogene Proteins,
pubmed-meshheading:8543805-Proto-Oncogene Proteins c-pim-1,
pubmed-meshheading:8543805-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:8543805-Proto-Oncogenes,
pubmed-meshheading:8543805-RNA, Messenger,
pubmed-meshheading:8543805-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:8543805-Receptors, Antigen, T-Cell,
pubmed-meshheading:8543805-Sezary Syndrome,
pubmed-meshheading:8543805-Signal Transduction,
pubmed-meshheading:8543805-Skin Neoplasms,
pubmed-meshheading:8543805-T-Lymphocytes,
pubmed-meshheading:8543805-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8543805-Transcription, Genetic,
pubmed-meshheading:8543805-Transfection,
pubmed-meshheading:8543805-Tumor Cells, Cultured
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pubmed:year |
1996
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pubmed:articleTitle |
pim-1 proto-oncogene expression in anti-CD3-mediated T cell activation is associated with protein kinase C activation and is independent of Raf-1.
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pubmed:affiliation |
Department of Veterans Affairs, Boise, ID 83702, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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