Linked Life Data

8541546

Source:http://linkedlifedata.com/resource/pubmed/id/8541546

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1996-2-13
pubmed:abstractText
Oligonucleotide primers for human interleukin-6 (IL-6) that bracketed the entire coding region of the gene were used in reverse transciptase-polymerase chain reaction (RT-PCR) studies to examine lL-6 expression in peripheral blood mononuclear cells (PBMC). In addition to the predicted 0.64-kb RT-PCR product, a second 0.45-kb product was observed. Cloning and dideoxy sequence analysis of this product revealed evidence for an alternatively spliced lL-6 transcript lacking exon II. Further RT-PCR analysis using forward primers ending at or one base before the exon I donor splice site again yielded both products. Additional primers were designed and successfully used to selectively distinguish the two forms of IL-6 transcript. Both transcripts were prominent in peripheral blood monocytes and lymphocytes, whereas only the 0.64-kb, full-length transcript was prominent in the lL-6-producing 5637 (human bladder carcinoma) cell line. Northern analysis revealed, in addition to the predominant 1.3-kb transcript, several minor transcripts at 1.9 to 4.8 kb that hybridized with the alternatively spliced cDNA probe but not with an exon II probe. Western analysis revealed lL-6 polypeptides of predicted size (26 to 29 kD) in culture medium from PBMC, while showing an immunoreactive band at 17 kD in cell lysates. These findings suggest the existence of an alternatively spliced form of lL-6 mRNA, which would encode for a polypeptide missing the gp130 interactive (signal-transducing) domain contained in exon II while retaining the lL-6 receptor (p80) domain. Such a molecule could in theory function as a natural antagonist of lL-6, as it would be expected to bind to the IL-6 receptor but not lead to signal transduction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4559-67
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8541546-Amino Acid Sequence, pubmed-meshheading:8541546-Antigens, CD, pubmed-meshheading:8541546-Base Sequence, pubmed-meshheading:8541546-Binding, Competitive, pubmed-meshheading:8541546-Carcinoma, pubmed-meshheading:8541546-DNA Primers, pubmed-meshheading:8541546-Exons, pubmed-meshheading:8541546-Gene Expression, pubmed-meshheading:8541546-Humans, pubmed-meshheading:8541546-Interleukin-6, pubmed-meshheading:8541546-Leukocytes, Mononuclear, pubmed-meshheading:8541546-Lymphocytes, pubmed-meshheading:8541546-Molecular Sequence Data, pubmed-meshheading:8541546-Monocytes, pubmed-meshheading:8541546-Neoplasm Proteins, pubmed-meshheading:8541546-Polymerase Chain Reaction, pubmed-meshheading:8541546-RNA, Messenger, pubmed-meshheading:8541546-RNA, Neoplasm, pubmed-meshheading:8541546-RNA Splicing, pubmed-meshheading:8541546-Receptors, Interleukin, pubmed-meshheading:8541546-Receptors, Interleukin-6, pubmed-meshheading:8541546-Sequence Alignment, pubmed-meshheading:8541546-Signal Transduction, pubmed-meshheading:8541546-Tumor Cells, Cultured, pubmed-meshheading:8541546-Urinary Bladder Neoplasms
pubmed:year
1995
pubmed:articleTitle
Detection and analysis of an alternatively spliced isoform of interleukin-6 mRNA in peripheral blood mononuclear cells.
pubmed:affiliation
Department of Medicine, University of Tennessee Medical Center at Knoxville 37920, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.