Linked Life Data

8529870

Source:http://linkedlifedata.com/resource/pubmed/id/8529870

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-1-26
pubmed:abstractText
A translational lacZ reporter system to study gene regulation in Neisseria gonorrhoeae (Ng) was developed. The pUC18-based vector pLES94 transforms Ng and recombines into the Ng chromosome at the site of the proAB genes. The vector contains a restriction site for cloning promoters that will result in a lacZ gene fusion. Initial cloning and characterization of promoters can be done in Escherichia coli. The vector contains both ApR and CmR genes, however the ApR gene is lost when the insert combines into the Ng chromosome. This system gives single copy expression of the fusion and does not result in the inactivation of the gene of interest.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-4
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Construction of a translational lacZ fusion system to study gene regulation in Neisseria gonorrhoeae.
pubmed:affiliation
Department of Microbiology and Immunology, University of Rochester Medical Center, NY 14642, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.