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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-1-30
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pubmed:databankReference |
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pubmed:abstractText |
Under negative superhelical stress, long (TG)n containing repeats experience a stepwise multiple B-Z transitions. We have investigated the effect of the plasmid size on this transitional behavior. A 66-bp (TG)n containing repeat from the 5'-untranscribed region of mouse ribosomal DNA was inserted in a 3-kb, a 6.5-kb and a 12.5-kb plasmids and its supercoil-driven B-Z transition was followed by OsO4 probing of topoisomer-populations. Our results show a clear correlation between the size of the topological domain and the extent of the region that converts cooperatively into Z-DNA at the initial transition.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0739-1102
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-56
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8527030-Animals,
pubmed-meshheading:8527030-Base Sequence,
pubmed-meshheading:8527030-DNA,
pubmed-meshheading:8527030-DNA, Ribosomal,
pubmed-meshheading:8527030-DNA, Superhelical,
pubmed-meshheading:8527030-Guanine,
pubmed-meshheading:8527030-Mice,
pubmed-meshheading:8527030-Molecular Sequence Data,
pubmed-meshheading:8527030-Mutagenesis, Insertional,
pubmed-meshheading:8527030-Nucleic Acid Conformation,
pubmed-meshheading:8527030-Particle Size,
pubmed-meshheading:8527030-Plasmids,
pubmed-meshheading:8527030-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8527030-Thymine
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pubmed:year |
1995
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pubmed:articleTitle |
The size of the topological domain modulates the B-Z transition of a (TG)n containing repeat.
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pubmed:affiliation |
Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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