Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 1
pubmed:dateCreated
1996-1-23
pubmed:abstractText
Protein translocation into the yeast endoplasmic reticulum requires the transport of ATP into the lumen of this organelle. Microsomal ATP transport activity was reconstituted into proteoliposomes to characterize and identify the transporter protein. A polypeptide was purified whose partial amino acid sequence demonstrated its identity to the product of the SAC1 gene. Accordingly, microsomal membranes isolated from strains harboring a deletion in the SAC1 gene (sac1 delta) were found to be deficient in ATP-transporting activity as well as severely compromised in their ability to translocate nascent prepro-alpha-factor and preprocarboxypeptidase Y. Proteins isolated from the microsomal membranes of a sac1 delta strain were incapable of stimulating ATP transport when reconstituted into the in vitro assay system. When immunopurified to homogeneity and incorporated into artificial lipid vesicles, Sac1p was shown to reconstitute ATP transport activity. Consistent with the requirement for ATP in the lumen of the ER to achieve the correct folding of secretory proteins, the sac1 delta strain was shown to have a severe defect in transport of procarboxypeptidase Y out of the ER and into the Golgi complex in vivo. The collective data indicate an intimate role for Sac1p in the transport of ATP into the ER lumen.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1327299, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1349170, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1356434, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1419037, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1568250, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1655273, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1731198, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1740446, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-1996357, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2183841, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2190988, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2215682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2449095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2544601, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2656401, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2661018, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2661019, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2687291, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2850167, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-2925690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-3000775, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-3009026, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-3072198, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-3512097, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-3517001, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-3790562, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-486419, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-6257680, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-6754086, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7017716, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7758110, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7790376, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7813006, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7923360, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7938015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-7961662, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8132492, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8253836, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8280473, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8294512, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8314848, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8440256, http://linkedlifedata.com/resource/pubmed/commentcorrection/8522598-8503045
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Antiporters, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Liposomes, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Monoester Hydrolases, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids, http://linkedlifedata.com/resource/pubmed/chemical/SAC1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/proteoliposomes
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1377-86
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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