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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-1-25
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pubmed:abstractText |
Bacterial isolates (including 17 Haemophilus somnus isolates and an H. somnus-like isolate) from asymptomatic or diseased cattle and sheep, were evaluated for markers associated with virulence and host predilection. The isolates were separated into 6 distinct biovariants, 3 for sheep and 3 for cattle, based on reactions in a battery of 21 test media. Three bovine isolates associated with disease caused hemolysis of bovine blood. The rest of the isolates did not hemolyze either bovine or ovine erythrocytes. Protein profiles of all H. somnus isolates were similar with the exception of the major outer membrane proteins (MOMPs). The MOMPs of isolates associated with disease in cattle had a relative molecular weight of approximately 41 kDa compared with 33 kDa for the MOMPs of isolates from asymptomatic cattle. The MOMPs from sheep isolates were either slightly higher or lower than the 41 kDa MOMPs of bovine isolates. Major antigens detected by Western blotting were similar in all isolates except the H. somnus-like isolate. An immunodominant 40 kDa antigen was conserved in all H. somnus isolates. Antibodies to this antigen have previously been found to be protective in cattle and may also be protective for sheep. Marked differences between cattle and sheep isolates were revealed by use of restriction enzyme analysis, which separated the isolates into 12 ribotypes and 15 unique DNA profiles. Thus, cattle and sheep isolates in this collection had distinctive differences in biochemical reactions, MOMP profiles, and DNA analyses. Such differences have potential value for epidemiological studies and may also be used to evaluate host specificity of H. somnus isolates.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-1212643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-13559617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-13853365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-1508038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-17422741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-17423184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-17423440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-2047597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-2245369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-2294052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-2379113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-2912900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3048622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3300006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3410539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3570472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3767100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3767797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3770951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-3984157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-4031034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-5749196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-6194548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-6408118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-6636461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-6833070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-7091841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-7690273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-7954123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-8385150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8521348-869799
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0830-9000
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-8
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8521348-Animals,
pubmed-meshheading:8521348-Blotting, Western,
pubmed-meshheading:8521348-Cattle,
pubmed-meshheading:8521348-Cattle Diseases,
pubmed-meshheading:8521348-DNA,
pubmed-meshheading:8521348-DNA Restriction Enzymes,
pubmed-meshheading:8521348-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8521348-Haemophilus,
pubmed-meshheading:8521348-Haemophilus Infections,
pubmed-meshheading:8521348-Sheep,
pubmed-meshheading:8521348-Sheep Diseases,
pubmed-meshheading:8521348-Virulence
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pubmed:year |
1995
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pubmed:articleTitle |
A comparative study of bovine and ovine Haemophilus somnus isolates.
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pubmed:affiliation |
University of Idaho, College of Agriculture, Caine Veterinary Teaching and Research Center, Caldwell, Idaho 83605, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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