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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1993-6-24
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pubmed:abstractText |
The effects of calcium ionophores A23187 and ionomycin on the c-myb and c-myc mRNA levels have been investigated in the Friend erythroleukemia cell line F4-6 using Northern blot analysis. Treatment of the cells with 0.5-4 microM A23187 or 1-4 microM ionomycin induced a concentration-dependent decrease in c-myb mRNA; this decrease was abolished by EGTA. c-myc mRNA levels were only moderately affected. After 12-24 h of calcium ionophore exposure, c-myb mRNA returned to pretreatment levels. No similar decrease in c-myb mRNA was seen with the sodium ionophore monensin (up to 16 microM). The dimethyl sulfoxide-induced suppression of c-myb and also of c-myc mRNA levels was not prevented in Ca(2+)-free medium and thus appeared Ca(2+)-independent. A23187 and ionomycin were capable of inducing beta-globin mRNA synthesis in F4-6 cells. Prolonged calcium ionophore exposure, however, strongly reduced cell viability and resulted only in a slight hemoglobin increase at lower concentrations. These results suggest that a rise in [Ca2+]i may be a signal leading to a transient decrease in c-myb mRNA and the initiation of erythroid differentiation in Friend cells. The transient suppression of c-myb mRNA levels represents a common feature of the action of dimethyl sulfoxide and calcium ionophores.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
268
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pubmed:geneSymbol |
c-myb,
c-myc
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10876-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8496152-Actins,
pubmed-meshheading:8496152-Animals,
pubmed-meshheading:8496152-Blotting, Northern,
pubmed-meshheading:8496152-Calcimycin,
pubmed-meshheading:8496152-Cell Survival,
pubmed-meshheading:8496152-Dimethyl Sulfoxide,
pubmed-meshheading:8496152-Egtazic Acid,
pubmed-meshheading:8496152-Friend murine leukemia virus,
pubmed-meshheading:8496152-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8496152-Genes, myc,
pubmed-meshheading:8496152-Hemoglobins,
pubmed-meshheading:8496152-Ionomycin,
pubmed-meshheading:8496152-Kinetics,
pubmed-meshheading:8496152-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8496152-Mice,
pubmed-meshheading:8496152-Oncogenes,
pubmed-meshheading:8496152-Proto-Oncogene Proteins,
pubmed-meshheading:8496152-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:8496152-RNA, Messenger,
pubmed-meshheading:8496152-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Calcium ionophore-induced transient down-regulation of c-myb mRNA levels in Friend erythroleukemia cells.
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pubmed:affiliation |
Department of Toxicology, Hamburg University Medical School, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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