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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1993-6-4
|
| pubmed:abstractText |
To analyze the relationship between c-myc gene expression and chondrocyte proliferation and maturation during endochondral ossification, Day 18-19 chick embryo sterna were pulse-labeled with [3H]thymidine, and serial sections were processed for autoradiography and in situ hybridization. Proliferating chondrocytes, located in four distinct areas of the developing sternum, all contained high levels of c-myc transcripts, whereas postmitotic chondrocytes (such as hypertrophic chondrocytes) contained undetectable amounts. These findings were confirmed by Northern blot analysis and by the observation that antisense c-myc oligomer treatment inhibited proliferation in cultured chondrocytes. Constitutive overexpression of c-myc by retroviral vectors in immature chondrocyte cultures (c-myc cultures) maintained the cells in a proliferative state and blocked their maturation into hypertrophic chondrocytes. The lack of maturation in the c-myc cultures was corroborated by analysis of type X collagen gene regulation. Control immature cultures contained strong repressor activity for the type X collagen gene promoter, as revealed by transfection assays; repressor activity was lost upon maturation and activation of type X collagen synthesis. In the c-myc cultures, however, repressor activity persisted. Thus, c-myc participates in the normal changes in proliferation accompanying chondrocyte maturation in vivo and in culture. The decreases in c-myc expression and cell proliferation appear to be required for completion of maturation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
268
|
| pubmed:geneSymbol |
c-myc,
v-myc
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9645-52
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8486652-Animals,
pubmed-meshheading:8486652-Autoradiography,
pubmed-meshheading:8486652-Base Sequence,
pubmed-meshheading:8486652-Blotting, Northern,
pubmed-meshheading:8486652-Cartilage,
pubmed-meshheading:8486652-Cell Division,
pubmed-meshheading:8486652-Cells, Cultured,
pubmed-meshheading:8486652-Chick Embryo,
pubmed-meshheading:8486652-Collagen,
pubmed-meshheading:8486652-DNA,
pubmed-meshheading:8486652-DNA Replication,
pubmed-meshheading:8486652-Exons,
pubmed-meshheading:8486652-Gene Expression,
pubmed-meshheading:8486652-Genes, myc,
pubmed-meshheading:8486652-In Situ Hybridization,
pubmed-meshheading:8486652-Kinetics,
pubmed-meshheading:8486652-Molecular Sequence Data,
pubmed-meshheading:8486652-Oligodeoxyribonucleotides,
pubmed-meshheading:8486652-Oligonucleotides, Antisense,
pubmed-meshheading:8486652-Osteogenesis,
pubmed-meshheading:8486652-Promoter Regions, Genetic,
pubmed-meshheading:8486652-RNA, Messenger,
pubmed-meshheading:8486652-Sternum,
pubmed-meshheading:8486652-Thionucleotides,
pubmed-meshheading:8486652-Thymidine,
pubmed-meshheading:8486652-Transcription, Genetic,
pubmed-meshheading:8486652-Tritium
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Expression and role of c-myc in chondrocytes undergoing endochondral ossification.
|
| pubmed:affiliation |
Department of Anatomy-Histology, University of Pennsylvania Dental School, Philadelphia 19104-6003.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|