Linked Life Data

8468476

Source:http://linkedlifedata.com/resource/pubmed/id/8468476

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8 Pt 1
pubmed:dateCreated
1993-5-12
pubmed:abstractText
Murine splenic B cells, when stimulated with LPS, show a generalized enhancement of gene transcription. In addition to this general increase, there is a specific increase in microseconds mRNA production and differentiation to high rate IgM secretion. Anti-mu added concomitantly with LPS at the start of culture has been demonstrated to inhibit the LPS-induced increase in microseconds mRNA production without affecting the proliferative capacity of the cells. By "run-on" analysis of nascent transcription, we have shown that the effect of anti-mu is mediated by the abrogation of the up-regulation of transcription of the mu-gene induced by LPS. Furthermore, by assessing the site of transcription termination, it is possible to infer that alterations in 3'-end processing induced by LPS are also inhibited. We have also found that CAT3 gene activity driven by a number of promoter/enhancers with diverse regulatory motifs are inhibited by anti-mu. These results suggest that the effect of anti-mu cannot be restricted to interactions with a single regulatory element. Therefore, cross-linking of surface IgM may affect a number of genes involved in differentiation to Ig secretion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3366-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Transcriptional analysis of inhibition of lipopolysaccharide response by anti-IgM.
pubmed:affiliation
University of Texas Southwestern Medical Center, Immunology Graduate Program, Dallas 75235.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't