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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1993-4-16
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pubmed:databankReference |
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pubmed:abstractText |
We describe a genomic DNA segment from spinach that bears part of the single-copy gene for ferredoxin-NADP(+)-oxidoreductase (FNR) including a 3.4 kb promoter sequence. Dissection of this DNA segment and its analysis in GUS (beta-glucuronidase) gene fusions in transgenic tobacco demonstrated that the promoter differs in structure from all other promoters for thylakoid protein genes studied to date. Two regions with light-responsive elements were identified. One is located within the first 118 bp upstream of the transcription initiation site. A second fragment covering nucleotide positions -220 to -119 is capable of conferring light-dependent GUS gene expression on two different minimal promoters. The latter fragment binds a transacting factor in gel-shift assays. None of the fragments carries cis elements known from other genes to be involved in light-controlled expression. Comparison of the light responsiveness of GUS gene fusions controlled by the -753/+231 and -118/+231 regions indicates that they respond differentially to phytochrome-dependent signals and that their expression in tobacco is not restricted to tissue with functional chloroplasts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
237
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
261-72
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8455561-Amino Acid Sequence,
pubmed-meshheading:8455561-Base Sequence,
pubmed-meshheading:8455561-Cloning, Molecular,
pubmed-meshheading:8455561-DNA Mutational Analysis,
pubmed-meshheading:8455561-Ferredoxin-NADP Reductase,
pubmed-meshheading:8455561-Gene Expression Regulation,
pubmed-meshheading:8455561-Genes, Plant,
pubmed-meshheading:8455561-Glucuronidase,
pubmed-meshheading:8455561-Histocytochemistry,
pubmed-meshheading:8455561-Light,
pubmed-meshheading:8455561-Molecular Sequence Data,
pubmed-meshheading:8455561-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:8455561-Plant Proteins,
pubmed-meshheading:8455561-Plants, Edible,
pubmed-meshheading:8455561-Plants, Genetically Modified,
pubmed-meshheading:8455561-Plants, Toxic,
pubmed-meshheading:8455561-Promoter Regions, Genetic,
pubmed-meshheading:8455561-RNA, Messenger,
pubmed-meshheading:8455561-Recombinant Proteins,
pubmed-meshheading:8455561-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8455561-Tobacco
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pubmed:year |
1993
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pubmed:articleTitle |
Characterization of the promoter from the single-copy gene encoding ferredoxin-NADP(+)-oxidoreductase from spinach.
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pubmed:affiliation |
Botanisches Institut, Ludwig-Maximilians-Universität, München, FRG.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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