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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1993-4-22
|
| pubmed:abstractText |
We have determined the patterns of mRNA and protein expression of 7 protein kinase C (PKC) isozymes in NIH 3T3 cells. Only PKC-alpha is expressed abundantly in NIH 3T3 cells; endogenous levels of the other 6 PKC isozymes are low or undetectable. We have overexpressed PKC-delta and -epsilon in these cells to observe activation/translocation of these two isozymes and the biological consequences of overexpression. Both PKC-delta and -epsilon, but not PKC-alpha, are partially associated with the insoluble fraction even in the absence of phorbol 12-myristate 13-acetate (PMA). Upon PMA stimulation, both PKC-delta and -epsilon translocate to the insoluble fraction of cell homogenates, as can be observed with the endogenous PKC-alpha. Overexpression of PKC-delta induces significant changes in morphology and causes the cells to grow more slowly and to a decreased cell density in confluent cultures. These changes are accentuated by treatment with PMA. Overexpression of PKC-epsilon does not lead to morphological changes, but causes increased growth rates and higher cell densities in monolayers. None of the PKC-delta overexpressers grow in soft agar with or without PMA, but all the cell lines that overexpress PKC-epsilon grow in soft agar in the absence of PMA, but not in its presence. NIH 3T3 cells that overexpress PKC-epsilon also form tumors in nude mice with 100% incidence. This indicates that high expression of PKC-epsilon contributes to neoplastic transformation.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide I
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6090-6
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:8454583-3T3 Cells,
pubmed-meshheading:8454583-Animals,
pubmed-meshheading:8454583-Biological Transport,
pubmed-meshheading:8454583-Blotting, Northern,
pubmed-meshheading:8454583-Blotting, Western,
pubmed-meshheading:8454583-Brain,
pubmed-meshheading:8454583-Cell Adhesion,
pubmed-meshheading:8454583-Cell Division,
pubmed-meshheading:8454583-Cell Transformation, Neoplastic,
pubmed-meshheading:8454583-Cloning, Molecular,
pubmed-meshheading:8454583-Enzyme Activation,
pubmed-meshheading:8454583-Enzyme Stability,
pubmed-meshheading:8454583-Indoles,
pubmed-meshheading:8454583-Isoenzymes,
pubmed-meshheading:8454583-Kinetics,
pubmed-meshheading:8454583-Maleimides,
pubmed-meshheading:8454583-Mice,
pubmed-meshheading:8454583-Mice, Inbred C57BL,
pubmed-meshheading:8454583-Mice, Nude,
pubmed-meshheading:8454583-Neoplasms, Experimental,
pubmed-meshheading:8454583-Protein Kinase C,
pubmed-meshheading:8454583-Tetradecanoylphorbol Acetate
|
| pubmed:year |
1993
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| pubmed:articleTitle |
Overexpression of protein kinase C-delta and -epsilon in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity.
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| pubmed:affiliation |
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
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| pubmed:publicationType |
Journal Article
|