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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1993-4-15
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pubmed:abstractText |
Hemoglobin synthesis in red cells is the major iron utilization pathway in the human body and accounts for > 80% of systemic iron turnover. The first step in erythroid heme biosynthesis is catalyzed by a tissue-specific isoform of 5-aminolevulinate synthase (ALAS). The previous identification of iron-responsive elements in the 5'-untranslated region of human and murine erythroid ALAS mRNA raised the intriguing possibility that eALAS expression might be under iron-dependent translational control. As a consequence, a single post-transcriptional regulatory system could coordinate cellular iron acquisition via the transferrin receptor, storage via ferritin, and utilization via eALAS. We directly demonstrate iron-dependent translational regulation of eALAS mRNA in murine erythroleukemia (MEL) cells. The iron-responsive element motif contained in eALAS mRNA is shown to be sufficient to confer translational control to a reporter mRNA both in transfected MEL cells and in vitro.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
268
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
5974-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8449958-5-Aminolevulinate Synthetase,
pubmed-meshheading:8449958-Animals,
pubmed-meshheading:8449958-Base Sequence,
pubmed-meshheading:8449958-Erythrocytes,
pubmed-meshheading:8449958-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8449958-Humans,
pubmed-meshheading:8449958-Iron,
pubmed-meshheading:8449958-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8449958-Mice,
pubmed-meshheading:8449958-Molecular Sequence Data,
pubmed-meshheading:8449958-Nucleic Acid Conformation,
pubmed-meshheading:8449958-Protein Biosynthesis,
pubmed-meshheading:8449958-RNA, Messenger,
pubmed-meshheading:8449958-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8449958-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Translational control of 5-aminolevulinate synthase mRNA by iron-responsive elements in erythroid cells.
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pubmed:affiliation |
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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