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rdf:type |
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|
lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1993-4-15
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pubmed:abstractText |
By in vitro transcription/translation of model proteins in the presence of dog pancreas microsomes, we have measured the minimum distance of an acceptor site from the lumenal end of a transmembrane segment required for N-linked glycosylation, both when the acceptor site is placed N- and C-terminally to the membrane anchor. We observe a sharp threshold at a distance of 12-14 residues, suggesting that the oligosaccharyltransferase active site is 30-40 A above the membrane and is oriented roughly parallel to the membrane surface.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5798-801
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8449946-Amino Acid Sequence,
pubmed-meshheading:8449946-Animals,
pubmed-meshheading:8449946-Base Sequence,
pubmed-meshheading:8449946-Binding Sites,
pubmed-meshheading:8449946-DNA,
pubmed-meshheading:8449946-Dogs,
pubmed-meshheading:8449946-Endopeptidases,
pubmed-meshheading:8449946-Endoplasmic Reticulum,
pubmed-meshheading:8449946-Escherichia coli,
pubmed-meshheading:8449946-Glycosylation,
pubmed-meshheading:8449946-Hexosyltransferases,
pubmed-meshheading:8449946-Intracellular Membranes,
pubmed-meshheading:8449946-Membrane Proteins,
pubmed-meshheading:8449946-Microsomes,
pubmed-meshheading:8449946-Molecular Sequence Data,
pubmed-meshheading:8449946-Pancreas,
pubmed-meshheading:8449946-Protein Conformation,
pubmed-meshheading:8449946-Serine Endopeptidases,
pubmed-meshheading:8449946-Transferases
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pubmed:year |
1993
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pubmed:articleTitle |
Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane.
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pubmed:affiliation |
Department of Molecular Biology, Karolinska Institute Center for Structural Biochemistry, NOVUM, Huddinge, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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