Linked Life Data

8449935

Source:http://linkedlifedata.com/resource/pubmed/id/8449935

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1993-4-15
pubmed:abstractText
Mitogenesis induced by most polypeptide growth factors is mediated by either G-protein- or tyrosine kinase-linked receptor pathways. Thrombin, a potent mitogen for vascular smooth muscle cells, activates a G-protein-coupled receptor but also requires tyrosine kinase activity for its mitogenic effect (Weiss, R. H., and Nuccitelli, R. (1992) J. Biol. Chem. 267, 5608-5613). Since this growth factor elicits a synergistic effect on DNA synthesis when applied to cells concurrently with basic fibroblast growth factor (bFGF), we suspected that the two growth factors have points of convergence in their signaling pathways. We now show that when 1 unit/ml thrombin is removed after an incubation period of from 4 h to 15 min prior to 15 ng/ml bFGF addition, its synergistic effect with bFGF on mitogenesis in vascular smooth muscle cells is preserved. Furthermore, appearance of bFGF in the cellular lysate is maximal 1 h after the addition of 1 unit/ml thrombin. While monoclonal antibody to bFGF inhibits thrombin's mitogenic effect by 63% at 30 micrograms/ml, it lacks an inhibitory effect on platelet-derived growth factor-BB-induced mitogenesis. The inhibitory effect of bFGF antibody on thrombin's growth is completely reversed by the addition of bFGF. These data demonstrate that the presence of bFGF is essential for thrombin to exert its full mitogenic effect in vascular smooth muscle cells, providing an example of a system where a tyrosine kinase-linked growth factor receptor system can act as an essential intermediary in the mitogenic signaling pathway of a G-protein-coupled receptor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5724-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
The mitogenic effect of thrombin in vascular smooth muscle cells is largely due to basic fibroblast growth factor.
pubmed:affiliation
Department of Internal Medicine, University of California School of Medicine, Davis 95616.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't