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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-3-24
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pubmed:abstractText |
Human thromboxane (TX) synthase (EC 5.3.99.5) was produced by the baculovirus expression system using cDNA encoding human TX synthase [(1991) Biochem. Biophys. Res. Commun. 78, 1479-1484]. A recombinant baculovirus TXS7 was expressed in Spodoptera frugiperda Sf9 insect cells. The expressed protein was recognized by monoclonal antibody, Kon 7 raised against human TX synthase [(1990) Blood 76, 80-85]. The recombinant TX synthase catalyzed the conversion of prostaglandin (PG) H2 to TXA2 and 12-hydroxy-heptadecatrienoic acid (HHT). Both conversions of PGH2 to TXA2 and HHT by the expressed TX synthase were completely inhibited by a specific TX synthase inhibitor, OKY-046 (5 microM).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
318
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
91-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8436233-Animals,
pubmed-meshheading:8436233-Baculoviridae,
pubmed-meshheading:8436233-Catalysis,
pubmed-meshheading:8436233-Cells, Cultured,
pubmed-meshheading:8436233-Cloning, Molecular,
pubmed-meshheading:8436233-DNA,
pubmed-meshheading:8436233-Fatty Acids, Unsaturated,
pubmed-meshheading:8436233-Humans,
pubmed-meshheading:8436233-Moths,
pubmed-meshheading:8436233-Prostaglandin H2,
pubmed-meshheading:8436233-Prostaglandins H,
pubmed-meshheading:8436233-Thromboxane-A Synthase,
pubmed-meshheading:8436233-Thromboxanes
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pubmed:year |
1993
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pubmed:articleTitle |
Expression of human thromboxane synthase using a baculovirus system.
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pubmed:affiliation |
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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