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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1993-3-23
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pubmed:abstractText |
The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low-molecular-mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169-182]. In the present work, medium-size sialylated oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC. From these fractions, the following six sialylated hepta- and octa-saccharide-alditols have been characterized by employing 500-MHz 1H-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy and methylation analysis. [formula: see text]
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Pronase,
http://linkedlifedata.com/resource/pubmed/chemical/Sugar Alcohols,
http://linkedlifedata.com/resource/pubmed/chemical/sialooligosaccharides
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
211
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
491-500
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:8436110-Bronchiectasis,
pubmed-meshheading:8436110-Carbohydrate Sequence,
pubmed-meshheading:8436110-Chromatography, Gel,
pubmed-meshheading:8436110-Chromatography, High Pressure Liquid,
pubmed-meshheading:8436110-Chromatography, Ion Exchange,
pubmed-meshheading:8436110-Glycoproteins,
pubmed-meshheading:8436110-Humans,
pubmed-meshheading:8436110-Hydrogen-Ion Concentration,
pubmed-meshheading:8436110-Magnetic Resonance Spectroscopy,
pubmed-meshheading:8436110-Methylation,
pubmed-meshheading:8436110-Molecular Sequence Data,
pubmed-meshheading:8436110-Mucus,
pubmed-meshheading:8436110-Oligosaccharides,
pubmed-meshheading:8436110-Pronase,
pubmed-meshheading:8436110-Sugar Alcohols
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pubmed:year |
1993
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pubmed:articleTitle |
Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis.
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pubmed:affiliation |
Unité des Protéines, Institut National de la Santé et de la Recherche Médicale, Lille, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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