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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-3-5
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pubmed:abstractText |
The structure of the G.G mispaired dodecanucleotide d(CGCGAATTGGCG)2 has been solved by x-ray crystallography and refined to an R factor of 18.8% at 2.2 A resolution for 3513 reflections. The dodecamer crystallizes as a B-type DNA double helix. It contains two G(anti).G(syn) base pairs--i.e., G-4/G-16(anti).G-21/G-9(syn). The Hoogsteen base pairing involves atoms O-6 and N-7 of the guanine in the syn conformation with atoms N-1 and N-2 of the anti-paired purine. One G.G base pair has a bifurcated hydrogen bond between G-4(N-1)...G-21(N-7) and G-4(N-1)...G-21(O-6). There is little overall structural distortion of the double helix induced as a consequence of the mispairing. The helical width is significantly increased by comparison with the structure of the native duplex, and the minor groove width in the 5'-AATT region is decreased. The G.G base pairing induces high-BII phosphate conformations at residues G-9 and T-20 in addition to more normal BII conformations at G-10 and G-22. It is suggested that these backbone aberrations provide signals for the facile repairability of G.G mispairs in DNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1518049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1545863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1545871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1606167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1660934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1708110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1812808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1837078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1930146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1934059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-1988677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2003222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2216754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2263612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2300578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2320109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2601741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-2695962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-3271438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-3304141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-3310237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-3690664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-3897553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-6273591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-6308634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-7174662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-7338904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8430089-958483
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
804-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1993
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pubmed:articleTitle |
Crystal structure of an oligonucleotide duplex containing G.G base pairs: influence of mispairing on DNA backbone conformation.
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pubmed:affiliation |
Cancer Research Campaign Biomolecular Structure Unit, Institute of Cancer Research, Sutton, Surrey, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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