Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-3-9
pubmed:databankReference
pubmed:abstractText
Glycoprotein IX is a relatively small (M(r) 20,000) surface glycoprotein of human platelets; one of three (Ib alpha, Ib beta, IX) polypeptide chains present in the glycoprotein Ib-IX complex that functions as the von Willebrand factor receptor and mediates platelet adhesion in the arterial circulation. Using a cDNA for human glycoprotein IX as the probe, clones were isolated from a human genomic library, and the genomic sequence for glycoprotein IX (3.2 kilobases) was determined. The transcriptional start site was located by RNase protection and primer extension experiments. The gene includes three exons and two introns within 1.6 kilobases of DNA, and the entire open reading frame for glycoprotein IX is included within the third exon. The genes for glycoproteins IX and Ib alpha share similar exon sequences on the 5' side of their ATG start codons, and both genes possess introns in this region. The glycoprotein IX gene contains two consensus regulatory sequences (GATA and ACTTCCT [ets]) in its promoter region (5' flank, within 67 bases of the start site) that are also present in similar sites in the previously described "megakaryocyte-platelet" genes (glycoprotein IIb, platelet factor 4, beta-thromboglobulin: human and rat). Thus, the glycoprotein IX gene shares structural features with other megakaryocyte-platelet genes and contains at least two consensus cis-acting regulatory elements that may govern gene expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3438-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8429020-Amino Acid Sequence, pubmed-meshheading:8429020-Base Sequence, pubmed-meshheading:8429020-Blood Platelets, pubmed-meshheading:8429020-Blotting, Southern, pubmed-meshheading:8429020-DNA, pubmed-meshheading:8429020-Exons, pubmed-meshheading:8429020-Genomic Library, pubmed-meshheading:8429020-Humans, pubmed-meshheading:8429020-Introns, pubmed-meshheading:8429020-Liver, pubmed-meshheading:8429020-Megakaryocytes, pubmed-meshheading:8429020-Molecular Sequence Data, pubmed-meshheading:8429020-Oligodeoxyribonucleotides, pubmed-meshheading:8429020-Open Reading Frames, pubmed-meshheading:8429020-Platelet Membrane Glycoproteins, pubmed-meshheading:8429020-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8429020-Restriction Mapping, pubmed-meshheading:8429020-Transcription, Genetic
pubmed:year
1993
pubmed:articleTitle
Characterization of the gene encoding human platelet glycoprotein IX.
pubmed:affiliation
Hematology Section, Seattle Veterans Administration Hospital, Washington.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't