Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-3-1
pubmed:databankReference
pubmed:abstractText
Idiotype (Id) 16/6 marks a variable (V) region structure that occurs frequently in the human immunoglobulin repertoire. The basis of the Id has been traced to a germline heavy chain gene segment, VH18/2 (VH26). To pursue the molecular basis for the frequency of Id 16/6, we have analyzed polymerase chain reaction-generated C mu, C gamma, and VH3 family V gene libraries derived from the circulating and tonsillar B cells of four normal individuals and from the B cells of two patients with active systemic lupus erythematosus (SLE). The frequency of VH18/2 in these libraries was compared with three control VH genes, VH56P1, VH21/28, and VHA57. Plaque lifts from C mu and C gamma VH cDNA libraries were screened with gene-specific oligonucleotide probes. The frequency of VH18/2 ranged from 4 to 10% of JH+ plaques (two of five times that of control VH genes). In four VH3 family-specific libraries derived from rearranged DNA, VH18/2 represented 19-33% of VH3+ plaques. Hybridizing VH18/2 plaques were 98-100% homologous to the germline VH gene; mutations when present were often in framework 3. Extensive variation was seen in the complementarity determining region 3 sequences of these rearranged V genes. The high frequency of VH18/2 expression in the B cell repertoire was confirmed by sequencing randomly picked JH+ plaques. In two patients with active SLE the frequency of use of VH18/2 was not greater than that observed in normal subjects. These results show that VH18/2 is overrepresented in the B cell repertoire of normal subjects and suggest that the immune repertoire may be dominated by relatively few V genes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1427920, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1537339, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1556192, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1649888, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1710233, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1730251, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1880429, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1899102, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1906675, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1908880, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1908883, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1909353, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1909646, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1971731, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-1976703, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2113307, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2117273, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2118157, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2120334, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2156935, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2317867, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2494263, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2503826, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-2562243, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3100050, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3104460, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3116084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3118465, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3298427, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3396540, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3487551, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3873490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-3874881, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-6092962, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-6198242, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-6450418, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-8459218, http://linkedlifedata.com/resource/pubmed/commentcorrection/8426111-8691047
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-18
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
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