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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-11-8
|
| pubmed:abstractText |
Polymorphonuclear leukocytes (PMNLs) are the most numerous cell population among the cellular infiltrates in gingival crevicular fluid (GCF) and play important roles in the host-defensive system in the gingival crevices. We determined the percentage of neutrophils, eosinophils and basophils in total PMNLs by light microscopic observation using Randolph-methylene blue staining, then assessed flow cytometric differences in the expression of CR3, Fc gamma RIII, Fc epsilon RII, LFA-1 alpha, and LFA-1 beta on PMNL in GCF and peripheral blood (PB) from 21 patients with adult periodontitis (AP) and 13 healthy donors. Percentages of basophils and eosinophils were higher in GCF than in PB. In both AP patients and healthy subjects, expression of CR3 and Fc epsilon RII was higher while Fc gamma RIII was lower in GCF than in PB. The statistical analysis showed that the expressions of Fc gamma RIII and Fc epsilon RII on GCF PMNLs were lower in AP patients than in healthy subjects. Expressions of LFA-1 alpha and beta on GCF were similar to those on PB PMNLs. PB PMNLs stimulated in vitro with Porphyromonas gingivalis culture supernatant and fMLP displayed an expression pattern of CR3, Fc gamma RIII and Fc epsilon RII on GCF PMNLs. However, C5a and IL-1 failed to induce changes in Fc gamma RIII and Fc epsilon RII. The results indicate that GCF neutrophils are activated, present enhanced adhesion and a decreased IgG-binding ability which would reflect that they are at the terminal stage of activation, and that GCF contains a larger eosinophil fraction than in PB. Moreover, these GCF eosinophils appear to be activated.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
D
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-3484
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
28
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
363-72
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8410601-Adult,
pubmed-meshheading:8410601-Analysis of Variance,
pubmed-meshheading:8410601-Blood Bactericidal Activity,
pubmed-meshheading:8410601-Culture Media, Conditioned,
pubmed-meshheading:8410601-Eosinophils,
pubmed-meshheading:8410601-Female,
pubmed-meshheading:8410601-Gingival Crevicular Fluid,
pubmed-meshheading:8410601-Humans,
pubmed-meshheading:8410601-Leukocyte Count,
pubmed-meshheading:8410601-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:8410601-Macrophage-1 Antigen,
pubmed-meshheading:8410601-Male,
pubmed-meshheading:8410601-Middle Aged,
pubmed-meshheading:8410601-Neutrophils,
pubmed-meshheading:8410601-Periodontitis,
pubmed-meshheading:8410601-Porphyromonas gingivalis,
pubmed-meshheading:8410601-Receptors, Fc,
pubmed-meshheading:8410601-Receptors, IgE,
pubmed-meshheading:8410601-Receptors, IgG,
pubmed-meshheading:8410601-Stimulation, Chemical
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Differential expression of CR3, Fc epsilon RII and Fc gamma RIII on polymorphonuclear leukocytes in gingival crevicular fluid.
|
| pubmed:affiliation |
Department of Periodontology, Niigata University School of Dentistry, Japan.
|
| pubmed:publicationType |
Journal Article
|