| pubmed-article:8408275 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C0041455 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C1440080 | lld:lifeskim |
| pubmed-article:8408275 | lifeskim:mentions | umls-concept:C0038891 | lld:lifeskim |
| pubmed-article:8408275 | pubmed:dateCreated | 1993-11-16 | lld:pubmed |
| pubmed-article:8408275 | pubmed:abstractText | During alveolar development and alveolar repair close contacts are established between fibroblasts and lung epithelial cells through gaps in the basement membrane. Using co-culture systems we have investigated whether these close contacts influence synthesis and secretion of the principal surfactant apoprotein (SP-A) by cultured rat lung alveolar type II cells and the synthesis and secretion of type I collagen by fibroblasts. The alveolar type II cells remained cuboidal and grew in colonies on fibroblast feeder layers and on Matrigel-coated cell culture inserts but were progressively more flattened on fixed fibroblast monolayers and plastic. Alveolar type II cells cultured on plastic released almost all their SP-A into the medium by 4 days. Alveolar type II cells cultured on viable fibroblasts or Matrigel-coated inserts above fibroblasts accumulated SP-A in the medium at a constant rate for the first 4 days, and probably recycle SP-A by endocytosis. The amount of mRNA for SP-A was very low after 4 days of culture of alveolar type II cells on plastic, Matrigel-coated inserts or fixed fibroblast monolayers: relatively, the amount of mRNA for SP-A was increased 4-fold after culture of alveolar type II cells on viable fibroblasts. Co-culture of alveolar type II cells with confluent human dermal fibroblasts stimulated by 2- to 3-fold the secretion of collagen type I into the culture medium, even after the fibroblasts' growth had been arrested with mitomycin C. Collagen secretion, by fibroblasts, also was stimulated 2-fold by conditioned medium from alveolar type II cells cultured on Matrigel. The amount of mRNA for type I collagen increased only modestly when fibroblasts were cultured in this conditioned medium. This stimulation of type I collagen secretion diminished as the conditioned medium was diluted out, but at high dilutions further stimulation occurred, indicating that a factor that inhibited collagen secretion also was being diluted out. The conditioned medium contained low levels of IGF-1 and the stimulation of type I collagen secretion was abolished when the conditioned medium was pre-incubated with antibodies to insulin-like growth factor 1 (IGF-1). There are important reciprocal interactions between alveolar type II cells and fibroblasts in co-culture. Direct contacts between alveolar type II cells and fibroblasts appear to have a trophic effect on cultured alveolar type II cells, increasing the levels of mRNA for SP-A. Rat lung alveolar type II cells appear to release a factor (possibly IGF-1) that stimulates type I collagen secretion by fibroblasts. | lld:pubmed |
| pubmed-article:8408275 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8408275 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8408275 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8408275 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8408275 | pubmed:issn | 0021-9533 | lld:pubmed |
| pubmed-article:8408275 | pubmed:author | pubmed-author:PowellJ TJT | lld:pubmed |
| pubmed-article:8408275 | pubmed:author | pubmed-author:GriffinMM | lld:pubmed |
| pubmed-article:8408275 | pubmed:author | pubmed-author:ChanY CYC | lld:pubmed |
| pubmed-article:8408275 | pubmed:author | pubmed-author:HamiltonGG | lld:pubmed |
| pubmed-article:8408275 | pubmed:author | pubmed-author:BhandariRR | lld:pubmed |
| pubmed-article:8408275 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8408275 | pubmed:volume | 105 ( Pt 2) | lld:pubmed |
| pubmed-article:8408275 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8408275 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8408275 | pubmed:pagination | 423-32 | lld:pubmed |
| pubmed-article:8408275 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:8408275 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8408275 | pubmed:articleTitle | Alveolar type II cell-fibroblast interactions, synthesis and secretion of surfactant and type I collagen. | lld:pubmed |
| pubmed-article:8408275 | pubmed:affiliation | Department of Biochemistry, Charing Cross and Westminister Medical School, London, UK. | lld:pubmed |
| pubmed-article:8408275 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8408275 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8408275 | lld:pubmed |
