Linked Life Data

8408275

Source:http://linkedlifedata.com/resource/pubmed/id/8408275

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-11-16
pubmed:abstractText
During alveolar development and alveolar repair close contacts are established between fibroblasts and lung epithelial cells through gaps in the basement membrane. Using co-culture systems we have investigated whether these close contacts influence synthesis and secretion of the principal surfactant apoprotein (SP-A) by cultured rat lung alveolar type II cells and the synthesis and secretion of type I collagen by fibroblasts. The alveolar type II cells remained cuboidal and grew in colonies on fibroblast feeder layers and on Matrigel-coated cell culture inserts but were progressively more flattened on fixed fibroblast monolayers and plastic. Alveolar type II cells cultured on plastic released almost all their SP-A into the medium by 4 days. Alveolar type II cells cultured on viable fibroblasts or Matrigel-coated inserts above fibroblasts accumulated SP-A in the medium at a constant rate for the first 4 days, and probably recycle SP-A by endocytosis. The amount of mRNA for SP-A was very low after 4 days of culture of alveolar type II cells on plastic, Matrigel-coated inserts or fixed fibroblast monolayers: relatively, the amount of mRNA for SP-A was increased 4-fold after culture of alveolar type II cells on viable fibroblasts. Co-culture of alveolar type II cells with confluent human dermal fibroblasts stimulated by 2- to 3-fold the secretion of collagen type I into the culture medium, even after the fibroblasts' growth had been arrested with mitomycin C. Collagen secretion, by fibroblasts, also was stimulated 2-fold by conditioned medium from alveolar type II cells cultured on Matrigel. The amount of mRNA for type I collagen increased only modestly when fibroblasts were cultured in this conditioned medium. This stimulation of type I collagen secretion diminished as the conditioned medium was diluted out, but at high dilutions further stimulation occurred, indicating that a factor that inhibited collagen secretion also was being diluted out. The conditioned medium contained low levels of IGF-1 and the stimulation of type I collagen secretion was abolished when the conditioned medium was pre-incubated with antibodies to insulin-like growth factor 1 (IGF-1). There are important reciprocal interactions between alveolar type II cells and fibroblasts in co-culture. Direct contacts between alveolar type II cells and fibroblasts appear to have a trophic effect on cultured alveolar type II cells, increasing the levels of mRNA for SP-A. Rat lung alveolar type II cells appear to release a factor (possibly IGF-1) that stimulates type I collagen secretion by fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
105 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-32
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8408275-Animals, pubmed-meshheading:8408275-Apoproteins, pubmed-meshheading:8408275-Basement Membrane, pubmed-meshheading:8408275-Cell Communication, pubmed-meshheading:8408275-Cells, Cultured, pubmed-meshheading:8408275-Collagen, pubmed-meshheading:8408275-Culture Media, Conditioned, pubmed-meshheading:8408275-Culture Media, Serum-Free, pubmed-meshheading:8408275-Epithelial Cells, pubmed-meshheading:8408275-Epithelium, pubmed-meshheading:8408275-Fibroblasts, pubmed-meshheading:8408275-Gene Expression Regulation, pubmed-meshheading:8408275-Humans, pubmed-meshheading:8408275-Insulin, pubmed-meshheading:8408275-Insulin-Like Growth Factor I, pubmed-meshheading:8408275-Intercellular Junctions, pubmed-meshheading:8408275-Mitomycin, pubmed-meshheading:8408275-Pulmonary Alveoli, pubmed-meshheading:8408275-Pulmonary Surfactant-Associated Proteins, pubmed-meshheading:8408275-Pulmonary Surfactants, pubmed-meshheading:8408275-RNA, Messenger, pubmed-meshheading:8408275-Rats
pubmed:year
1993
pubmed:articleTitle
Alveolar type II cell-fibroblast interactions, synthesis and secretion of surfactant and type I collagen.
pubmed:affiliation
Department of Biochemistry, Charing Cross and Westminister Medical School, London, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't