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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1993-11-16
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pubmed:abstractText |
We have isolated genetic suppressors of mutations in the recJ gene of Escherichia coli in a locus we term srjA. These srjA mutations cause partial to complete alleviation of the recombination and UV repair defects conferred by recJ153 and recJ154 mutations in a recBC sbcA genetic background. The srjA gene was mapped to 37.5 min on the E. coli chromosome. This chromosomal region from the srjA5 strain was cloned into a plasmid vector and was shown to confer recJ suppression in a dominant fashion. Mutational analysis of this plasmid mapped srjA to the infC gene encoding translation initiation factor 3 (IF3). Sequence analysis revealed that all three srjA alleles cause amino acid substitutions of IF3. Suppression of recJ was shown to be allele specific: recJ153 and recJ154 mutations were suppressible, but recJ77 and the insertion allele recJ284::Tn10 were not. In addition, growth medium-conditional lethality was observed for strains carrying srjA mutations with the nonsuppressible recJ alleles. When introduced into recJ+ strains, srjA mutations conferred hyperrecombinational and hyper-UVr phenotypes. An interesting implication of these genetic properties of srjA suppression is that IF3 may regulate the expression of recJ and perhaps other recombination genes and hence may regulate the recombinational capacity of the cell.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-14294081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-1987126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2194094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2648393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2649886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2670665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2695390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2991205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-2993230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-3041370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-3298224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-4587405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-4884815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-5330239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-5338813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6099322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6251971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6254853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6257642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6317649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6317865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6337999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6340723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6353409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-6390429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-790387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8407784-8483930
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
175
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pubmed:geneSymbol |
infC,
recB,
recC,
recJ,
sbcA,
srjA
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6118-25
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8407784-Alleles,
pubmed-meshheading:8407784-Bacterial Proteins,
pubmed-meshheading:8407784-Base Sequence,
pubmed-meshheading:8407784-Conjugation, Genetic,
pubmed-meshheading:8407784-DNA Primers,
pubmed-meshheading:8407784-Dose-Response Relationship, Radiation,
pubmed-meshheading:8407784-Escherichia coli,
pubmed-meshheading:8407784-Eukaryotic Initiation Factor-3,
pubmed-meshheading:8407784-Genes, Bacterial,
pubmed-meshheading:8407784-Genotype,
pubmed-meshheading:8407784-Kinetics,
pubmed-meshheading:8407784-Molecular Sequence Data,
pubmed-meshheading:8407784-Mutation,
pubmed-meshheading:8407784-Peptide Initiation Factors,
pubmed-meshheading:8407784-Plasmids,
pubmed-meshheading:8407784-Polymerase Chain Reaction,
pubmed-meshheading:8407784-Recombination, Genetic,
pubmed-meshheading:8407784-Restriction Mapping,
pubmed-meshheading:8407784-Suppression, Genetic,
pubmed-meshheading:8407784-Transduction, Genetic,
pubmed-meshheading:8407784-Ultraviolet Rays
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pubmed:year |
1993
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pubmed:articleTitle |
Suppression of recJ mutations of Escherichia coli by mutations in translation initiation factor IF3.
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pubmed:affiliation |
Rosensteil Basic Medical Sciences Center, Brandeis University, Waltham, Massachusetts 02254-9110.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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