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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-6-8
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pubmed:abstractText |
Mitogen stimulation of T cells in vitro has been employed in the analysis of the T cell antigen receptor (TCR) repertoire and as a method of generating T cell lines and clones. It has been suspected for some time that mitogen stimulation may bias the repertoire. We have addressed this problem employing a semi-quantitative technique utilizing the polymerase chain reaction (PCR) and flow cytometry. Using this PCR method and a panel of primers to 22 V beta subgroups, the V beta repertoire of both unstimulated and phytohaemagglutinin (PHA)-stimulated peripheral T cells from eight healthy individuals was investigated. The samples were also analysed by flow cytometry using anti-V beta 2, V beta 5 and V beta 8 MoAbs. A significant increase in the expression of V beta 6, V beta 7.2 and V beta 10.1 was found in all eight samples of PHA-stimulated T cells compared with unstimulated T cells using the PCR method. In contrast, no differences were found between unstimulated and PHA-stimulated T cells by flow cytometry. These results question the validity of using mitogen-stimulated T cells to investigate TCR gene usage.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1311263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1347855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1348030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1466748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1530851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1533355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1672649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1675173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1685492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1829139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1829410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1832875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1836200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1857971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1940794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-1971424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-2011599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-2148526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-2197981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-2424978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-2524876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-3261760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-3876513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-3934668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387412-6600491
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
361-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8387412-Antigens, CD3,
pubmed-meshheading:8387412-Base Sequence,
pubmed-meshheading:8387412-Flow Cytometry,
pubmed-meshheading:8387412-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:8387412-Humans,
pubmed-meshheading:8387412-Lymphocyte Activation,
pubmed-meshheading:8387412-Mitogens,
pubmed-meshheading:8387412-Molecular Sequence Data,
pubmed-meshheading:8387412-Oligodeoxyribonucleotides,
pubmed-meshheading:8387412-Polymerase Chain Reaction,
pubmed-meshheading:8387412-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8387412-T-Lymphocytes
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pubmed:year |
1993
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pubmed:articleTitle |
The human T cell receptor V beta repertoire of normal peripheral blood lymphocytes before and after mitogen stimulation.
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pubmed:affiliation |
Department of Medicine, King's College School of Medicine and Dentistry, Denmark Hill, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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