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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4 Pt 1
|
| pubmed:dateCreated |
1993-5-17
|
| pubmed:abstractText |
Ca2+/calmodulin (CaM)-dependent protein kinase II is a major effector of the Ca2+ signaling pathway. It has a wide tissue distribution and phosphorylates multiple substrates. Villus enterocytes from rat ileum contain a Ca2+/CaM-dependent kinase activity that phosphorylates the exogenous neural substrate synapsin I. This phosphorylation is blocked by a specific peptide inhibitor. Antibodies made to rat brain Ca2+/CaM-dependent protein kinase II label a single band with a relative molecular mass of approximately 50 kDa in isolated rat enterocytes by immunoblot. Almost one-half of this immunoreactive protein is preferentially found in a particulate compared with a soluble subcellular fraction of the enterocytes. Virtually all of the 50-kDa band in the particulate fraction is insoluble in nonionic detergent, suggesting that the kinase is associated with the enterocyte cytoskeleton. Antibodies to Ca2+/CaM-dependent protein kinase II immunocytochemically detect fibrillar structures concentrated in the terminal web region of intestinal epithelial cells that colocalized with myosin II. This enzyme may have a role in regulating the intestinal epithelial cytoskeleton.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
C1029-36
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:8386447-Animals,
pubmed-meshheading:8386447-Antibodies,
pubmed-meshheading:8386447-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:8386447-Cell Fractionation,
pubmed-meshheading:8386447-Epithelial Cells,
pubmed-meshheading:8386447-Epithelium,
pubmed-meshheading:8386447-Fluorescent Antibody Technique,
pubmed-meshheading:8386447-Ileum,
pubmed-meshheading:8386447-Immunoblotting,
pubmed-meshheading:8386447-Immunohistochemistry,
pubmed-meshheading:8386447-Male,
pubmed-meshheading:8386447-Peptide Mapping,
pubmed-meshheading:8386447-Phosphopeptides,
pubmed-meshheading:8386447-Phosphorylation,
pubmed-meshheading:8386447-Protein Kinases,
pubmed-meshheading:8386447-Rats,
pubmed-meshheading:8386447-Rats, Sprague-Dawley,
pubmed-meshheading:8386447-Subcellular Fractions
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Distribution of calcium/calmodulin-dependent protein kinase II in rat ileal enterocytes.
|
| pubmed:affiliation |
Department of Surgery, Department of Veterans Affairs Hospital, West Haven, Connecticut 06516.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|